ANXA7 Recombinant Rabbit Monoclonal Antibody [JE65-36]
cat.: HA721078
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE65-36 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 53 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human ANXA7 aa 1-250/488. |
| Positive control: | SH-SY5Y cell lysates, rat brain tissue lysates. |
| Subcellular location: | Endoplasmic reticulum membrane, extracellular exosome, nucleus, collagen-containing extracellular matrix, cytoplasm, membrane. |
| Recommended Dilutions:
WB |
1:500 |
| Uniprot #: | SwissProt: P20073 Human Entrez Gene: 155423 Rat |
| Alternative names: | Annexin A7 Annexin VII Annexin-7 ANX7 ANXA7 ANXA7_HUMAN SNX Synexin |
Images
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Fig1:
Western blot analysis of ANXA7 on different lysates with Rabbit anti-ANXA7 antibody (HA721078) at 1/1,000 dilution. Lane 1: A549-WT cell lysate Lane 2: A549-KD ANXA7 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721078) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of ANXA7 on SH-SY5Y cell lysates with Rabbit anti-ANXA7 antibody (HA721078) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721078) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Western blot analysis of ANXA7 on rat brain tissue lysates with Rabbit anti-ANXA7 antibody (HA721078) at 1/500 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721078) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.