NDUFS2 Recombinant Rabbit Monoclonal Antibody [JE65-29]
cat.: HA721079
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE65-29 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 53 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human NDUFS2 aa 264-463/463. |
| Positive control: | Jurkat cell lysate, 293T cell lysate, A431 cell lysate, Raji cell lysate, K-562 cell lysate, RAW264.7 cell lysate, PC-12 cell lysate, Mouse brain tissue lysate, Mouse kidney tissue lysate, Rat brain tissue lysate, Rat kidney tissue lysate, human kidney tissue. |
| Subcellular location: | Mitochondrion inner membrane. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:100 |
| Uniprot #: | SwissProt: O75306 Human | Q91WD5 Mouse | Q641Y2 Rat |
| Alternative names: | CI 49 CI 49kD CI-49kD Complex 1, mitochondrial respiratory chain, 49 KD subunit Complex I 49kD Complex I 49kDa subunit Complex I-49kD mitochondrial NADH dehydrogenase (ubiquinone) Fe S protein 2 49kDa NADH dehydrogenase (ubiquinone) Fe S protein 2, 49kDa (NADH coenzyme Q reductase) NADH dehydrogenase [ubiquinone] iron sulfur protein 2, mitochondrial NADH dehydrogenase [ubiquinone] iron-sulfur protein 2 NADH ubiquinone oxidoreductase 49 kDa subunit NADH ubiquinone oxidoreductase NDUFS2 subunit NADH-ubiquinone oxidoreductase 49 kDa subunit NADH:ubiquinone oxidoreductase core subunit S2 Ndufs2 NDUS2_HUMAN |
Images
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Fig1:
Western blot analysis of NDUFS2 on different lysates with Rabbit anti-NDUFS2 antibody (HA721079) at 1/2,000 dilution. Lane 1: Jurkat cell lysate (20 µg/Lane) Lane 2: 293T cell lysate (20 µg/Lane) Lane 3: A431 cell lysate (20 µg/Lane) Lane 4: Raji cell lysate (20 µg/Lane) Lane 5: K-562 cell lysate (20 µg/Lane) Lane 6: RAW264.7 cell lysate (20 µg/Lane) Lane 7: PC-12 cell lysate (20 µg/Lane) Lane 8: Mouse brain tissue lysate (40 µg/Lane) Lane 9: Mouse kidney tissue lysate (40 µg/Lane) Lane 10: Rat brain tissue lysate (40 µg/Lane) Lane 11: Rat kidney tissue lysate (40 µg/Lane) Predicted band size: 53 kDa Observed band size: 45 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721079) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-NDUFS2 antibody (HA721079) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721079) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.