Homer1 Recombinant Rabbit Monoclonal Antibody [JE65-19]
cat.: HA721081
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat, Cynomolgus monkey, Pig |
| Applications: | WB, IHC-P, IHC-Fr |
| Clonality: | Monoclonal |
| Clone number: | JE65-19 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 40 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Homer1 aa 101-250/354. |
| Positive control: | Mouse brain tissue lysate, Rat brain tissue lysate, mouse brain tissue, mouse hippocampus tissue, rat cerebellum tissue. |
| Subcellular location: | Cytoplasm, postsynaptic density, synapse, dendritic spine. |
| Recommended Dilutions:
WB IHC-P IHC-Fr |
1:20,000 1:500 1:500 |
| Uniprot #: | SwissProt: Q9Z2Y3 Mouse | Q9Z214 Rat |
| Alternative names: | HOME1_HUMAN Homer HOMER 1A HOMER 1B HOMER 1C Homer homolog 1 (Drosophila) homer homolog 1 Homer protein homolog 1 homer scaffolding protein 1 Homer, Drosophila, homolog of, 1 Homer, neuronal immediate early gene, 1 Homer-1 Homer1 HOMER1A HOMER1B HOMER1C SYN47 Ves 1 |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required |
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Fig2:
Application: IHC-Fr Species: Rat Site: Cerebellum Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required |
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Fig3:
Western blot analysis of Homer1 on different lysates with Rabbit anti-Homer1 antibody (HA721081) at 1/20,000 dilution. Lane 1: Mouse brain tissue lysate (20 µg/Lane) Lane 2: Mouse lung tissue lysate (low expression) (20 µg/Lane) Lane 3: Rat brain tissue lysate (20 µg/Lane) Lane 4: Rat lung tissue lysate (low expression) (20 µg/Lane) Predicted band size: 40 kDa Observed band size: 46 kDa Exposure time: 8 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721081) at 1/20,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. Low expression: heart, kidney and lung (PMID: 19046353). |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Homer1 antibody (HA721081) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721081) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-Homer1 antibody (HA721081) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721081) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-Homer1 antibody (HA721081) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721081) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.