EMC4 Recombinant Rabbit Monoclonal Antibody [JE65-17]
cat.: HA721082
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE65-17 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 20 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human GRSF1 aa 21-70/183. |
| Positive control: | 293 cell lysate, HepG2 cell lysate, SH-SY5Y cell lysate, U87MG cell lysates, rat brain tissue lysate, rat cerebellum tissue lysate, mouse brain tissue lysate, rat brain tissue, rat cerebellum tissue, mouse brain tissue. |
| Subcellular location: | Endoplasmic reticulum membrane. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:5,000 1:100-1:400 |
| Uniprot #: | SwissProt: Q5J8M3 Human | Q9CZX9 Mouse Entrez Gene: 296049 Rat |
| Alternative names: | Cell proliferation-inducing gene 17 protein EMC4 ER membrane protein complex subunit 4 HSPC184 PIG17 tmem85 TMM85_HUMAN Transmembrane protein 85 |
Images
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Fig1:
Western blot analysis of EMC4 on different lysates with Rabbit anti-EMC4 antibody (HA721082) at 1/500 dilution. Lane 1: 293 cell lysate Lane 2: HepG2 cell lysate Lane 3: SH-SY5Y cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 20 kDa Observed band size: 18 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721082) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of EMC4 on U87MG cell lysates with Rabbit anti-EMC4 antibody (HA721082) at 1/5,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 20 kDa Observed band size: 18 kDa Exposure time: 30 seconds; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721082) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Western blot analysis of EMC4 on different lysates with Rabbit anti-EMC4 antibody (HA721082) at 1/500 dilution. Lane 1: Rat brain tissue lysate Lane 2: Rat cerebellum tissue lysate Lane 3: Mouse brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 20 kDa Observed band size: 18 kDa Exposure time: 1 minute; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721082) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-EMC4 antibody (HA721082) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721082) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-EMC4 antibody (HA721082) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721082) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig6:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-EMC4 antibody (HA721082) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721082) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.