SNX5 Recombinant Rabbit Monoclonal Antibody [JE64-96]
cat.: HA721084
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE64-96 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 47 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human SNX5 aa 1-150/404. |
| Positive control: | Mouse spleen tissue lysate, rat spleen tissue lysate, Jurkat cell lysate, 293T cell lysate, Hela cell lysate, HepG2 cell lysate, rat kidney tissue, human lymph nodes tissue, human thyroid carcinoma tissue, mouse kidney tissue. |
| Subcellular location: | Cytoplasm, Endosome, Early endosome, Early endosome membrane, Cell membrane, Cytoplasmic vesicle membrane, phagocytic cup, ruffle. |
| Recommended Dilutions:
WB IHC-P |
1:500 1:100-1:400 |
| Uniprot #: | SwissProt: Q9Y5X3 Human | Q9D8U8 Mouse | B1H267 Rat |
| Alternative names: | 0910001N05Rik 1810032P22Rik AU019504 D2Ertd52e FLJ10931 OTTHUMP00000030340 OTTHUMP00000062927 OTTMUSP00000003367 OTTMUSP00000003368 RP11-504H3.2 RP23-35E16.2 snoRNA MBI-43 SNX 5 SNX5 SNX5_HUMAN Sorting nexin 5 Sorting nexin-5 |
Images
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Fig1:
Western blot analysis of SNX5 on different lysates with Rabbit anti-SNX5 antibody (HA721084) at 1/500 dilution. Lane 1: Mouse spleen tissue lysate Lane 2: Rat spleen tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 47 kDa Observed band size: 50 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721084) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of SNX5 on different lysates with Rabbit anti-SNX5 antibody (HA721084) at 1/500 dilution. Lane 1: Jurkat cell lysate Lane 2: 293T cell lysate Lane 3: Hela cell lysate Lane 4: HepG2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 47 kDa Observed band size: 50 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721084) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-SNX5 antibody (HA721084) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721084) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-SNX5 antibody (HA721084) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721084) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma tissue with Rabbit anti-SNX5 antibody (HA721084) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721084) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-SNX5 antibody (HA721084) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721084) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.