SNX5 Recombinant Rabbit Monoclonal Antibody [JE64-96]
cat.: HA721084
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE64-96 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 47 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human SNX5 aa 1-150/404. |
| Positive control: | Mouse spleen tissue lysate, Rat spleen tissue lysate, Jurkat cell lysate, 293T cell lysate, Hela cell lysate, HepG2 cell lysate, human lymph nodes tissue, human thyroid carcinoma tissue, mouse kidney tissue, rat kidney tissue. |
| Subcellular location: | Cytoplasm, Endosome, Early endosome, Early endosome membrane, Cell membrane, Cytoplasmic vesicle membrane, phagocytic cup, ruffle. |
| Recommended Dilutions:
WB IHC-P |
1:5000 1:100-1:400 |
| Uniprot #: | SwissProt: Q9Y5X3 Human | Q9D8U8 Mouse | B1H267 Rat |
| Alternative names: | 0910001N05Rik 1810032P22Rik AU019504 D2Ertd52e FLJ10931 OTTHUMP00000030340 OTTHUMP00000062927 OTTMUSP00000003367 OTTMUSP00000003368 RP11-504H3.2 RP23-35E16.2 snoRNA MBI-43 SNX 5 SNX5 SNX5_HUMAN Sorting nexin 5 Sorting nexin-5 |
Images
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Fig1:
Western blot analysis of SNX5 on different lysates with Rabbit anti-SNX5 antibody (HA721084) at 1/5,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 2: Jurkat (Human T-lymphoblastic cells) cell lysate Lane 3: RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate Lane 4: C6 (Rat glioma cell) cell lysate Lane 5: Mouse spleen tissue lysate Lane 6: Rat spleen tissue lysate Lysates/proteins at 15 µg/Lane. Exposure time: 180 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA721084, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 47 kDa Observed band size: 47 kDa |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-SNX5 antibody (HA721084) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721084) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma tissue with Rabbit anti-SNX5 antibody (HA721084) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721084) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-SNX5 antibody (HA721084) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721084) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-SNX5 antibody (HA721084) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721084) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.