TCEA1 Recombinant Rabbit Monoclonal Antibody [JE64-90]
cat.: HA721086
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE64-90 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 34 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human TCEA1 aa 201-250/301. |
| Positive control: | K562 cell lysate, HUVEC cell lysate, Hela cell lysate, Jurkat cell lysate, mouse pancreas tissue lysates, rat pancreas tissue lysates, human lung cancer tissue, mouse colon tissue, rat colon tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:5,000 1:1,000 |
| Uniprot #: | SwissProt: P23193 Human | P10711 Mouse | Q4KLL0 Rat |
| Alternative names: | GTF2S SII TCEA Tcea1 TCEA1_HUMAN TF2S TFIIS Transcription elongation factor A (SII) 1 Transcription elongation factor A protein 1 Transcription elongation factor A, 1 Transcription elongation factor S-II protein 1 Transcription elongation factor TFIIS.o |
Images
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Fig1:
Western blot analysis of TCEA1 on different lysates with Rabbit anti-TCEA1 antibody (HA721086) at 1/500 dilution. Lane 1: K562 cell lysate Lane 2: HUVEC cell lysate Lane 3: Hela cell lysate Lane 4: Jurkat cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 34 kDa Observed band size: 37 kDa Exposure time: 1 minute; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721086) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of TCEA1 on mouse pancreas tissue lysates with Rabbit anti-TCEA1 antibody (HA721086) at 1/2,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 34 kDa Observed band size: 37 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721086) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Western blot analysis of TCEA1 on rat pancreas tissue lysates with Rabbit anti-TCEA1 antibody (HA721086) at 1/5,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 34 kDa Observed band size: 37 kDa Exposure time: 1 minute 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721086) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-TCEA1 antibody (HA721086) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721086) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-TCEA1 antibody (HA721086) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721086) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-TCEA1 antibody (HA721086) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721086) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.