PNKP Recombinant Rabbit Monoclonal Antibody [JE64-85]
cat.: HA721087
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE64-85 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 57 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human PNKP aa 460-509/521. |
| Positive control: | HepG2 cell lysate, 293 cell lysate, PC-3 cell lysate, Jurkat cell lysate, human colon carcinoma tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:500 1:100 |
| Uniprot #: | SwissProt: Q96T60 Human |
| Alternative names: | 2''(3'')-polynucleotidase 2'(3')-polynucleotidase Bifunctional polynucleotide phosphatase/kinase DEM 1 DEM1 DNA 5' kinase/3' phosphatase DNA 5''-kinase/3''-phosphatase EIEE10 Homo sapiens polynucleotide kinase 3' phosphatase (PNKP) MCSZ PNK 1 PNK1 Pnkp PNKP DNA kinase PNKP_HUMAN Polynucleotide 3'-phosphatase Polynucleotide 5' hydroxyl kinase Polynucleotide 5''-hydroxyl-kinase Polynucleotide kinase 3 prime phosphatase Polynucleotide kinase 3' phosphatase Polynucleotide Kinase Polynucleotide kinase-3''-phosphatase |
Images
|
Fig1:
Western blot analysis of PNKP on different lysates with Rabbit anti-PNKP antibody (HA721087) at 1/500 dilution. Lane 1: HepG2 cell lysate Lane 2: 293 cell lysate Lane 3: PC-3 cell lysate Lane 4: Jurkat cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 57 kDa Observed band size: 60 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721087) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-PNKP antibody (HA721087) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721087) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.