PNKP Recombinant Rabbit Monoclonal Antibody [JE64-85]
cat.: HA721087
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE64-85 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 57 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human PNKP aa 460-509/521. |
| Positive control: | HepG2 cell lysate, Jurkat cell lysate, A549 cell lysate, Hela cell lysate, human colon carcinoma tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:5000 1:100 |
| Uniprot #: | SwissProt: Q96T60 Human |
| Alternative names: | 2''(3'')-polynucleotidase 2'(3')-polynucleotidase Bifunctional polynucleotide phosphatase/kinase DEM 1 DEM1 DNA 5' kinase/3' phosphatase DNA 5''-kinase/3''-phosphatase EIEE10 Homo sapiens polynucleotide kinase 3' phosphatase (PNKP) MCSZ PNK 1 PNK1 Pnkp PNKP DNA kinase PNKP_HUMAN Polynucleotide 3'-phosphatase Polynucleotide 5' hydroxyl kinase Polynucleotide 5''-hydroxyl-kinase Polynucleotide kinase 3 prime phosphatase Polynucleotide kinase 3' phosphatase Polynucleotide Kinase Polynucleotide kinase-3''-phosphatase |
Images
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Fig1:
Western blot analysis of PNKP on different lysates with Rabbit anti-PNKP antibody (HA721087) at 1/5,000 dilution. Lane 1: HepG2 (Human liver cancer cell) cell lysate Lane 2: Jurkat (Human T-lymphoblastic cells) cell lysate Lane 3: A549 (Human lung adenocarcinoma cell) cell lysate Lane4: Hela (Human cervical adenocarcinoma cell) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA721087, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 57.1 kDa Observed band size: 60 kDa |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-PNKP antibody (HA721087) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721087) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.