NSDHL Recombinant Rabbit Monoclonal Antibody [JE64-86]
cat.: HA721088
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE64-86 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 42 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human NSDHL aa 151-200/373. |
| Positive control: | HeLa cell lysate, HepG2 cell lysate, A431 cell lysate, HEK-293 cell lysate, AGS cell lysate, MCF7 cell lysate, MDA-MB-231 cell lysate, Human liver tissue lysate, human liver tissue, human colon carcinoma tissue, Hela. |
| Subcellular location: | Endoplasmic reticulum membrane, Lipid droplet. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:1,000-1:5,000 1:200 1:400 |
| Uniprot #: | SwissProt: Q15738 Human |
| Alternative names: | decarboxylating H105E3 H105e3 protein NAD(P) dependent steroid dehydrogenase like NSDHL NSDHL_HUMAN Protein H105e3 SDR31E1 Short chain dehydrogenase/reductase family 31E member 1 Sterol 4 alpha carboxylate 3 dehydrogenase decarboxylating Sterol-4-alpha-carboxylate 3-dehydrogenase XAP104 |
Images
|
Fig1:
Western blot analysis of NSDHL on different lysates with Rabbit anti-NSDHL antibody (HA721088) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: A431 cell lysate Lane 4: HEK-293 cell lysate Lane 5: AGS cell lysate Lane 6: MCF7 cell lysate Lane 7: MDA-MB-231 cell lysate Lane 8: Human liver tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 42 kDa Observed band size: 37 kDa Exposure time: 3 minutes 22 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721088) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of NSDHL on different lysates with Rabbit anti-NSDHL antibody (HA721088) at 1/5,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-NSDHL KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 42 kDa Observed band size: 37 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721088) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-NSDHL antibody (HA721088) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721088) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-NSDHL antibody (HA721088) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721088) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunocytochemistry analysis of Hela cells labeling NSDHL with Rabbit anti-NSDHL antibody (HA721088) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-NSDHL antibody (HA721088) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta Ⅲ tubulin (M0805-8, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) were used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.