PRPF31 Recombinant Rabbit Monoclonal Antibody [JE64-82]
cat.: HA721089
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JE64-82 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 55 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human PRPF31 aa 350-399/499. |
| Positive control: | Jurkat cell lysates, HepG2 cell lysates, human skin tissue, human esophagus tissue, SiHa, human colon carcinoma tissue. |
| Subcellular location: | Nucleus, Nucleus speckle, Cajal body. |
| Recommended Dilutions:
WB IHC-P FC |
1:500 1:200-1:1,500 1:500-1:1,000 |
| Uniprot #: | SwissProt: Q8WWY3 Human |
| Alternative names: | DKFZp566J153 hPrp 31 hPrp31 NY BR 99 Pre mRNA processing factor 31 Pre mRNA processing factor 31 homolog (yeast) Pre mRNA processing factor 31 homolog Pre-mRNA-processing factor 31 Precursor mRNA-processing factor 31, S. cerevisiae, homolog of Protein 61K PRP 31 PRP31 PRP31 pre mRNA processing factor 31 homolog (yeast) PRP31 pre mRNA processing factor 31 homolog PRP31_HUMAN PRPF 31 prpf31 RP 11 RP11 Serologically defined breast cancer antigen NY BR 99 Serologically defined breast cancer antigen NY-BR-99 SNRNP61 U4/U6 small nuclear ribonucleoprotein Prp31 U4/U6 snRNP 61 kDa protein |
Images
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Fig1:
Western blot analysis of PRPF31 on Jurkat cell lysates with Rabbit anti-PRPF31 antibody (HA721089) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 55 kDa Observed band size: 60 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721089) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of PRPF31 on HepG2 cell lysates with Rabbit anti-PRPF31 antibody (HA721089) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 55 kDa Observed band size: 60 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721089) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-PRPF31 antibody (HA721089) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721089) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human esophagus tissue with Rabbit anti-PRPF31 antibody (HA721089) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721089) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Flow cytometric analysis of SiHa cells labeling PRPF31. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721089, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig6:
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-PRPF31 antibody (HA721089) at 1/1,500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721089) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.