GNE Recombinant Rabbit Monoclonal Antibody [JE64-81]
cat.: HA721090
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE64-81 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 79 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human GNE aa 673-722/722. |
| Positive control: | SW480 cell lysate, A549 cell lysate, Hela cell lysate. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB |
1:500 |
| Uniprot #: | SwissProt: Q9Y223 Human |
| Alternative names: | 2310066H07Rik Bifunctional UDP N acetylglucosamine 2 epimerase/N acetylmannosamine kinase DMRV GLCNE_HUMAN Glucosamine (UDP N acetyl) 2 epimerase/N acetylmannosamine kinase GNE IBM2 ManAc kinase N acylmannosamine kinase N-acetylmannosamine kinase NM RP23-209M8.6 Uae1 UDP GlcNAc 2 epimerase UDP GlcNAc 2 epimerase/ManAc kinase UDP N acetylglucosamine 2 epimerase/N acetylmannosamine kinase UDP-GlcNAc-2-epimerase UDP-GlcNAc-2-epimerase/ManAc kinase Uridine diphosphate N acetylglucosamine 2 epimerase Uridine diphosphate-N-acetylglucosamine-2-epimerase |
Images
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Fig1:
Western blot analysis of GNE on different lysates with Rabbit anti-GNE antibody (HA721090) at 1/500 dilution. Lane 1: SW480 cell lysate Lane 2: A549 cell lysate Lane 3: Hela cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 79 kDa Observed band size: 79 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721090) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.