GNE Recombinant Rabbit Monoclonal Antibody [JE64-81]
cat.: HA721090
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE64-81 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 79 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human GNE aa 673-722/722. |
| Positive control: | A549 cell lysate, HeLa cell lysate, Mouse liver tissue lysate, Rat liver tissue lysate, rat liver tissue. |
| Subcellular location: | Cytoplasm, cytosol. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:200 |
| Uniprot #: | SwissProt: Q9Y223 Human | Q91WG8 Mouse | O35826 Rat |
| Alternative names: | 2310066H07Rik Bifunctional UDP N acetylglucosamine 2 epimerase/N acetylmannosamine kinase DMRV GLCNE_HUMAN Glucosamine (UDP N acetyl) 2 epimerase/N acetylmannosamine kinase GNE IBM2 ManAc kinase N acylmannosamine kinase N-acetylmannosamine kinase NM RP23-209M8.6 Uae1 UDP GlcNAc 2 epimerase UDP GlcNAc 2 epimerase/ManAc kinase UDP N acetylglucosamine 2 epimerase/N acetylmannosamine kinase UDP-GlcNAc-2-epimerase UDP-GlcNAc-2-epimerase/ManAc kinase Uridine diphosphate N acetylglucosamine 2 epimerase Uridine diphosphate-N-acetylglucosamine-2-epimerase |
Images
|
Fig1:
Western blot analysis of GNE on different lysates with Rabbit anti-GNE antibody (HA721090) at 1/1,000 dilution. Lane 1: A549 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: Mouse liver tissue lysate (40 µg/Lane) Lane 4: Rat liver tissue lysate (40 µg/Lane) Predicted band size: 79 kDa Observed band size: 71 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721090) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-GNE antibody (HA721090) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721090) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.