BRAT1 Recombinant Rabbit Monoclonal Antibody [JE64-71]
cat.: HA721091
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE64-71 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 88 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human BRAT1 aa 672-821/821. |
| Positive control: | MCF-7 cell lysate, PC-3 cell lysate, 293 cell lysate, K562 cell lysate, A431 cell lysate, THP-1 cell lysate, A549 cell lysate. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB |
1:500 |
| Uniprot #: | SwissProt: Q6PJG6 Human |
| Alternative names: | BAAT 1 BAAT1 BRAT 1 brat1 BRAT1_HUMAN BRCA1-associated ATM activator 1 BRCA1-associated protein required for ATM activation protein 1 BRCA1-associated protein required for ATM activation-1 C7orf27 chromosome 7 open reading frame 27 HEAT repeat containing protein C7orf27 MGC22916 RMFSL |
Images
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Fig1:
Western blot analysis of BRAT1 on different lysates with Rabbit anti-BRAT1 antibody (HA721091) at 1/500 dilution. Lane 1: MCF-7 cell lysate Lane 2: PC-3 cell lysate Lane 3: 293 cell lysate Lane 4: K562 cell lysate Lane 5: A431 cell lysate Lane 6: THP-1 cell lysate Lane 7: A549 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 88 kDa Observed band size: 77 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721091) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.