BRAT1 Recombinant Rabbit Monoclonal Antibody [JE64-71]
cat.: HA721091
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JE64-71 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 88 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human BRAT1 aa 672-821/821. |
| Positive control: | MCF-7 cell lysate, PC-3 cell lysate, 293 cell lysate, K562 cell lysate, A431 cell lysate, THP-1 cell lysate, A549 cell lysate, HeLa. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IF-Cell |
1:500 1:100 |
| Uniprot #: | SwissProt: Q6PJG6 Human |
| Alternative names: | BAAT 1 BAAT1 BRAT 1 brat1 BRAT1_HUMAN BRCA1-associated ATM activator 1 BRCA1-associated protein required for ATM activation protein 1 BRCA1-associated protein required for ATM activation-1 C7orf27 chromosome 7 open reading frame 27 HEAT repeat containing protein C7orf27 MGC22916 RMFSL |
Images
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Fig1:
Western blot analysis of BRAT1 on different lysates with Rabbit anti-BRAT1 antibody (HA721091) at 1/500 dilution. Lane 1: MCF-7 cell lysate Lane 2: PC-3 cell lysate Lane 3: 293 cell lysate Lane 4: K562 cell lysate Lane 5: A431 cell lysate Lane 6: THP-1 cell lysate Lane 7: A549 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 88 kDa Observed band size: 77 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721091) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of HeLa cells labeling BRAT1 with Rabbit anti-BRAT1 antibody (HA721091) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-BRAT1 antibody (HA721091) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.