GALT Recombinant Rabbit Monoclonal Antibody [JE64-66]
cat.: HA721092
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE64-66 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 43 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human GALT aa 41-90/379. |
| Positive control: | Hela cell lysate, HepG2 cell lysate, rat brain tissue lysate, mouse kidney tissue lysate, rat bladder tissue, mouse large intestine tissue, human kidney tissue. |
| Subcellular location: | Cytosol, Golgi apparatus, cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:100-1:400 |
| Uniprot #: | SwissProt: P07902 Human | Q03249 Mouse | P43424 Rat |
| Alternative names: | Gal 1 P uridylyltransferase Gal-1-P uridylyltransferase Galactose 1 phosphate uridyl transferase Galactose 1 phosphate uridylyltransferase Galactose-1-phosphate uridylyltransferase GALT GALT_HUMAN UDP glucose hexose 1 phosphate uridylyltransferase UDP-glucose--hexose-1-phosphate uridylyltransferase |
Images
|
Fig1:
Western blot analysis of GALT on different lysates with Rabbit anti-GALT antibody (HA721092) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-GALT KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 43 kDa Observed band size: 43 kDa Exposure time: 62 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721092) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded rat bladder tissue with Rabbit anti-GALT antibody (HA721092) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721092) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse large intestine tissue with Rabbit anti-GALT antibody (HA721092) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721092) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-GALT antibody (HA721092) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721092) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Western blot analysis of GALT on Hela cell lysate with Rabbit anti-GALT antibody (HA721092) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Exposure time: 62 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA721092, 1/1,000 in primary antibody dilution buffer 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 43.4 kDa Observed band size: 43 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.