MST4 Recombinant Rabbit Monoclonal Antibody [JE64-64]
cat.: HA721094
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB
Clonality: Monoclonal
Clone number: JE64-64
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 47 kDa
Isotype: IgG
Immunogen: Recombinant protein within human MST4 aa aa 317-416/416.
Positive control: HEK-293 cell lysate, RD cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Mouse small intestine tissue lysate.
Subcellular location: Golgi apparatus, Cytoplasm.
Recommended Dilutions:
  WB

1:2,000
Uniprot #: SwissProt: Q9P289 Human | Q99JT2 Mouse
Entrez Gene: 317589 Rat
Alternative names: 2610018G03Rik EC 2.7.11.1 Mammalian Ste20 like protein kinase 4 Mammalian STE20-like protein kinase 4 Mammalian sterile 20 like 4 MASK MST 4 MST-4 MST3 and SOK1 related kinase Mst3 and SOK1-related kinase Mst4 MST4_HUMAN RGD1563568 RP23-245F8.1 RP6 213H19.1 Serine/threonine protein kinase 26 Serine/threonine protein kinase MASK Serine/threonine protein kinase MST 4 Serine/threonine protein kinase MST4 Serine/threonine-protein kinase MASK Serine/threonine-protein kinase MST4 STE20 like kinase MST 4 STE20 like kinase MST4 STE20-like kinase MST4 STK26
Images
HA721094_1.jpg Fig1: Western blot analysis of MST4 on different lysates with Rabbit anti-MST4 antibody (HA721094) at 1/2,000 dilution.

Lane 1: HEK-293 cell lysate
Lane 2: RD cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: PC-12 cell lysate
Lane 5: Mouse small intestine tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 47 kDa
Observed band size: 47 kDa

Exposure time: Lane 1-4: 51 seconds; Lane 5: 15 seconds; ECL: K1802;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721094) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.