MST4 Recombinant Rabbit Monoclonal Antibody [JE64-64]
cat.: HA721094
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE64-64 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 47 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human MST4 aa aa 317-416/416. |
| Positive control: | Jurkat cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, MCF-7 cell lysate, rat skeletal muscle tissue, human prostate tissue, human small intestine tissue, mouse cerebellum tissue. |
| Subcellular location: | Golgi apparatus, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:500 1:400-1:1,000 |
| Uniprot #: | SwissProt: Q9P289 Human | Q99JT2 Mouse Entrez Gene: 317589 Rat |
| Alternative names: | 2610018G03Rik EC 2.7.11.1 Mammalian Ste20 like protein kinase 4 Mammalian STE20-like protein kinase 4 Mammalian sterile 20 like 4 MASK MST 4 MST-4 MST3 and SOK1 related kinase Mst3 and SOK1-related kinase Mst4 MST4_HUMAN RGD1563568 RP23-245F8.1 RP6 213H19.1 Serine/threonine protein kinase 26 Serine/threonine protein kinase MASK Serine/threonine protein kinase MST 4 Serine/threonine protein kinase MST4 Serine/threonine-protein kinase MASK Serine/threonine-protein kinase MST4 STE20 like kinase MST 4 STE20 like kinase MST4 STE20-like kinase MST4 STK26 |
Images
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Fig1:
Western blot analysis of MST4 on different lysates with Rabbit anti-MST4 antibody (HA721094) at 1/500 dilution. Lane 1: Jurkat cell lysate Lane 2: NIH/3T3 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 47 kDa Observed band size: 50 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721094) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of MST4 on different lysates with Rabbit anti-MST4 antibody (HA721094) at 1/500 dilution. Lane 1: PC-12 cell lysate Lane 2: MCF-7 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 47 kDa Observed band size: 50 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721094) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-MST4 antibody (HA721094) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721094) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human prostate tissue with Rabbit anti-MST4 antibody (HA721094) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721094) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-MST4 antibody (HA721094) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721094) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig6:
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-MST4 antibody (HA721094) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721094) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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