CYFIP1 Recombinant Rabbit Monoclonal Antibody [JE64-11]
cat.: HA721095
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE64-11 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 145 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human CYFIP1 aa 51-100/1,253. |
| Positive control: | SW480 cell lysate, RAW264.7 cell lysate, rat lung tissue lysate, NIH/3T3 cell lysate, rat lung tissue lysates, rat esophagus tissue, rat stomach tissue. |
| Subcellular location: | Cytoplasm, perinuclear region, lamellipodium, ruffle, synaptosome. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:100-1:400 |
| Uniprot #: | SwissProt: Q7L576 Human | Q7TMB8 Mouse Entrez Gene: 308666 Rat |
| Alternative names: | CY CYFIP 1 CYFIP1 CYFP1_HUMAN Cytoplasmic FMR1 interacting protein 1 Cytoplasmic FMR1-interacting protein 1 FLJ45151 KIAA0068 p140sra 1 p140sra-1 P140SRA1 Selective hybridizing clone SHYC Specifically Rac1 associated protein 1 Specifically Rac1-associated protein 1 SRA 1 Sra-1 SRA1 |
Images
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Fig1:
Western blot analysis of CYFIP1 on different lysates with Rabbit anti-CYFIP1 antibody (HA721095) at 1/1,000 dilution. Lane 1: SW480 cell lysate Lane 2: RAW264.7 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 145 kDa Observed band size: 130 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721095) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of CYFIP1 on different lysates with Rabbit anti-CYFIP1 antibody (HA721095) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-CYFIP1 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 145 kDa Observed band size: 130 kDa Exposure time: 180 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721095) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Western blot analysis of CYFIP1 on different lysates with Rabbit anti-CYFIP1 antibody (HA721095) at 1/500 dilution. Lane 1: Rat lung tissue lysate (20 µg/Lane) Lane 2: NIH/3T3 cell lysate (10 µg/Lane) Lysates/proteins at 10 µg/Lane. Predicted band size: 145 kDa Observed band size: 130 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721095) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig4:
Western blot analysis of CYFIP1 on rat lung tissue lysates with Rabbit anti-CYFIP1 antibody (HA721095) at 1/500 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 145 kDa Observed band size: 130 kDa Exposure time: 30 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721095) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat esophagus tissue with Rabbit anti-CYFIP1 antibody (HA721095) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721095) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded rat stomach tissue with Rabbit anti-CYFIP1 antibody (HA721095) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721095) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.