RAB45 Recombinant Rabbit Monoclonal Antibody [JE65-33]
cat.: HA721097
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JE65-33 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 83 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human RAB45 aa 81-130/740. |
| Positive control: | K562 cell lysate, A431 cell lysate, RAW264.7 cell lysate, NIH/3T3 cell lysate, rat large intestine tissue, human thyroid tissue, Hela. |
| Subcellular location: | Perinuclear region. |
| Recommended Dilutions:
WB IHC-P FC |
1:500 1:100 1:500-1:1,000 |
| Uniprot #: | SwissProt: Q8IZ41 Human | Q5RI75 Mouse Entrez Gene: 298138 Rat |
| Alternative names: | FLJ31614 RAB45 RAB45 member RAS oncogene family RAS and EF hand domain containing RAS and EF hand domain containing protein Ras related protein Rab 45 |
Images
|
Fig1:
Western blot analysis of RAB45 on different lysates with Rabbit anti-RAB45 antibody (HA721097) at 1/500 dilution. Lane 1: K562 cell lysate Lane 2: A431 cell lysate Lane 3: RAW264.7 cell lysate Lane 4: NIH/3T3 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 83 kDa Observed band size: 85 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721097) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded rat large intestine tissue with Rabbit anti-RAB45 antibody (HA721097) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721097) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human thyroid tissue with Rabbit anti-RAB45 antibody (HA721097) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721097) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Flow cytometric analysis of Hela cells labeling RAB45. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721097, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.