eIF1A Recombinant Rabbit Monoclonal Antibody [JE64-63]
cat.: HA721099
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE64-63 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 16 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human eIF1A aa 51-100/144. |
| Positive control: | Jurkat cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, human colon carcinoma tissue. |
| Subcellular location: | Cytosol. |
| Recommended Dilutions:
WB IHC-P |
1:500 1:400 |
| Uniprot #: | SwissProt: P47813 Human | Q8BMJ3 Mouse | Q6VV72 Rat |
| Alternative names: | eIF-1A eIF-1A X isoform eIF-4C EIF1A EIF1AP1 EIF1AX EIF4C eukaryotic translation initiation factor 1A X-linked eukaryotic translation initiation factor 1A, X chromosome Eukaryotic translation initiation factor 1A, X-chromosomal eukaryotic translation initiation factor 4C Putative eukaryotic translation initiation factor 1A |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-eIF1A antibody (HA721099) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721099) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2:
Western blot analysis of eIF1A on different lysates with Rabbit anti-eIF1A antibody (HA721099) at 1/1,000 dilution. Lane 1: Jurkat (Human T-lymphoblastic cells) cell lysate Lane 2: HepG2 (Human hepatocellular carcinoma cell) cell lysate Lane 3: NIH/3T3 (Mouse fibroblast) cell lysate Lane 4: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 4 seconds ; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA721099, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 16 kDa Observed band size: 16 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.