eIF1A Recombinant Rabbit Monoclonal Antibody [JE64-63]
cat.: HA721099
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: JE64-63
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 16 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human eIF1A aa 51-100/144.
Positive control: Jurkat cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, human colon carcinoma tissue.
Subcellular location: Cytosol.
Recommended Dilutions:
  WB
  IHC-P

1:500
1:400
Uniprot #: SwissProt: P47813 Human | Q8BMJ3 Mouse | Q6VV72 Rat
Alternative names: eIF-1A eIF-1A X isoform eIF-4C EIF1A EIF1AP1 EIF1AX EIF4C eukaryotic translation initiation factor 1A X-linked eukaryotic translation initiation factor 1A, X chromosome Eukaryotic translation initiation factor 1A, X-chromosomal eukaryotic translation initiation factor 4C Putative eukaryotic translation initiation factor 1A
Images
HA721099_1.jpg Fig1: Western blot analysis of eIF1A on different lysates with Rabbit anti-eIF1A antibody (HA721099) at 1/500 dilution.

Lane 1: Jurkat cell lysate
Lane 2: HepG2 cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: PC-12 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 16 kDa
Observed band size: 16 kDa

Exposure time: 2 minutes;

15% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721099) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature."
HA721099_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-eIF1A antibody (HA721099) at 1/400 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721099) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.