RAVER2 Recombinant Rabbit Monoclonal Antibody [JE64-36]
cat.: HA721100
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: JE64-36
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 74 kDa
Isotype: IgG
Immunogen: Recombinant protein within human RAVER2 aa 442-691/691.
Positive control: 293T cell lysates, human cervix carcinoma tissue.
Subcellular location: Cytoplasm, Nucleus.
Recommended Dilutions:
  WB
  IHC-P

1:1,000
1:400
Uniprot #: SwissProt: Q9HCJ3 Human
Alternative names: DKFZp762D1011 FLJ10770 KIAA1579 Protein raver 2 Protein raver-2 Raver2 RAVR2_HUMAN Ribonucleoprotein PTB binding 2 Ribonucleoprotein PTB-binding 2
Images
HA721100_1.jpg Fig1: Western blot analysis of RAVER2 on 293T cell lysates with Rabbit anti-RAVER2 antibody (HA721100) at 1/1,000 dilution.

Lysates/proteins at 10 µg/Lane.2

Predicted band size: 74 kDa
Observed band size: 74 kDa

Exposure time: 2 minutes;

10% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721100) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
HA721100_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human cervix carcinoma tissue with Rabbit anti-RAVER2 antibody (HA721100) at 1/400 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721100) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.