hnRNP G-T Recombinant Rabbit Monoclonal Antibody [JE64-33]
cat.: HA721104
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P
Clonality: Monoclonal
Clone number: JE64-33
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 43 kDa
Isotype: IgG
Immunogen: Recombinant protein within human hnRNP G-T aa 151-350/392.
Positive control: 293 cell lysate, Hela cell lysate, rat testis tissue lysates, Hela, human testis tissue, mouse testis tissue.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
1:500-1:5,000
1:200
1:400
Uniprot #: SwissProt: O75526 Human | Q9DAE2 Mouse
Entrez Gene: 365344 Rat
Alternative names: Heterogeneous nuclear ribonucleoprotein G T hnRNP G T hnRNP G-T HNRNPG T HNRNPGT HNRPGT RBMX L2 RBMXL 2 RBMXL2 RMXL2_HUMAN RNA binding motif protein X linked like 2 RNA-binding motif protein Testes specific heterogenous nuclear ribonucleoprotein G T Testis-specific heterogeneous nuclear ribonucleoprotein G-T X-linked-like-2
Images
HA721104_1.jpg Fig1: Western blot analysis of hnRNP G-T on different lysates with Rabbit anti-hnRNP G-T antibody (HA721104) at 1/5,000 dilution.

Lane 1: 293 cell lysate
Lane 2: Hela cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 43 kDa
Observed band size: 43 kDa

Exposure time: 2 minutes;

12% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721104) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
HA721104_2.jpg Fig2: Western blot analysis of hnRNP G-T on rat testis tissue lysates with Rabbit anti-hnRNP G-T antibody (HA721104) at 1/500 dilution.

Lysates/proteins at 20 µg/Lane.

Predicted band size: 43 kDa
Observed band size: 43 kDa

Exposure time: 2 minutes;

10% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721104) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
HA721104_3.jpg Fig3: Immunocytochemistry analysis of Hela cells labeling hnRNP G-T with Rabbit anti-hnRNP G-T antibody (HA721104) at 1/200 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-hnRNP G-T antibody (HA721104) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

Beta Ⅲ tubulin (M0805-8, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) were used as the secondary antibody at 1/1,000 dilution.
HA721104_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-hnRNP G-T antibody (HA721104) at 1/400 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721104) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721104_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-hnRNP G-T antibody (HA721104) at 1/400 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721104) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.