HA721105

RPS8 Recombinant Rabbit Monoclonal Antibody [JE65-57]

cat.: HA721105

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IF-Cell, IHC-P, FC
Clonality:	Monoclonal
Clone number:	JE65-57

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 24 kDa
Isotype:	IgG
Immunogen:	Recombinant protein within human RPS8 aa 1-208/208.
Positive control:	Daudi cell lysate, mouse liver tissue lysate, mouse brain tissue lysate, rat testis tissue lysate, THP-1 cell lysate, 293 cell lysate, SH-SY5Y, rat cerebellum tissue, human placenta tissue, mouse lung tissue, THP-1.
Subcellular location:	Cytoplasm, Membrane.
Recommended Dilutions: WB IF-Cell IHC-P FC	1:500-1:1,000 1:400 1:400 1:500-1:1,000
Uniprot #:	SwissProt: P62241 Human \| P62242 Mouse \| P62243 Rat
Alternative names:	40S ribosomal protein S8 OK/SW-cl.83 OTTHUMP00000010160 OTTHUMP00000010161 Ribosomal protein S8 RPS8 RS8_HUMAN S8 "

Images

Fig1: Western blot analysis of RPS8 on different lysates with Rabbit anti-RPS8 antibody (HA721105) at 1/500 dilution.

Lane 1: Daudi cell lysate, 10 µg/Lane
Lane 2: Mouse liver tissue lysate, 20 µg/Lane

Predicted band size: 24 kDa
Observed band size: 25 kDa

Exposure time: 2 minutes;

12% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721105) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

Fig2: Western blot analysis of RPS8 on different lysates with Rabbit anti-RPS8 antibody (HA721105) at 1/1,000 dilution.

Lane 1: Mouse brain tissue lysate
Lane 2: Rat testis tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 24 kDa
Observed band size: 25 kDa

Exposure time: 2 minutes;

15% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721105) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

Fig3: Western blot analysis of RPS8 on different lysates with Rabbit anti-RPS8 antibody (HA721105) at 1/1,000 dilution.

Lane 1: THP-1 cell lysate
Lane 2: 293 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 24 kDa
Observed band size: 25 kDa

Exposure time: 1 minute;

15% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721105) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

Fig4: Immunocytochemistry analysis of SH-SY5Y cells labeling RPS8 with Rabbit anti-RPS8 antibody (HA721105) at 1/400 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RPS8 antibody (HA721105) at 1/400 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) were used as the secondary antibody at 1/1,000 dilution.

	Fig5: Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-RPS8 antibody (HA721105) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721105) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig6: Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-RPS8 antibody (HA721105) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721105) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig7: Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-RPS8 antibody (HA721105) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721105) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig8: Flow cytometric analysis of THP-1 cells labeling RPS8.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA721105, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.