NTH1 Recombinant Rabbit Monoclonal Antibody [JE65-02]
cat.: HA721108
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE65-02 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 34 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human NTH1 aa 1-150/312. |
| Positive control: | Hela cell lysate, 293 cell lysate, K562 cell lysate, human kidney tissue. |
| Subcellular location: | Mitochondrion, Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:2,000 1:400 |
| Uniprot #: | SwissProt: P78549 Human |
| Alternative names: | Bifunctional DNA N glycoslyase/DNA (apurinic or apyrimidinic site) lyase DNA glycoslyase/AP lyase Endonuclease III like protein 1 Endonuclease III-like protein 1 hNTH1 NTH 1 nth endonuclease III like 1 (E. coli) NTH endonuclease III Like 1 NTH1 NTHL 1 Nthl1 NTHL1_HUMAN OCTS 3 OCTS3 |
Images
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Fig1:
Western blot analysis of NTH1 on different lysates with Rabbit anti-NTH1 antibody (HA721108) at 1/1,000 dilution. Lane 1: Hela cell lysate Lane 2: 293 cell lysate Lane 3: K562 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 34 kDa Observed band size: 36 kDa Exposure time: 1 minute; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721108) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of NTH1 on different lysates with Rabbit anti-NTH1 antibody (HA721108) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-NTH1 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 34 kDa Observed band size: 36 kDa Exposure time: 12 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721108) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-NTH1 antibody (HA721108) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721108) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.