PGM1 Recombinant Rabbit Monoclonal Antibody [JE65-06]
cat.: HA721110
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE65-06 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 61 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human PGM1 aa 513-562/562. |
| Positive control: | 293T cell lysate, A431 cell lysate, Hela cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, PC-12 cell lysate, rat spleen tissue lysate, mouse skeletal muscle tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:500 1:100 |
| Uniprot #: | SwissProt: P36871 Human | Q9D0F9 Mouse | P38652 Rat |
| Alternative names: | CDG1T Glucose phosphomutase 1 GSD14 OTTHUMP00000010519 OTTHUMP00000010520 PGM 1 PGM1 PGM1_HUMAN Phosphoglucomutase 1 Phosphoglucomutase-1 Phosphoglucomutase1 |
Images
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Fig1:
Western blot analysis of PGM1 on different lysates with Rabbit anti-PGM1 antibody (HA721110) at 1/500 dilution. Lane 1: 293T cell lysate, 10 µg/Lane Lane 2: A431 cell lysate, 10 µg/Lane Lane 3: Hela cell lysate, 10 µg/Lane Lane 4: HepG2 cell lysate, 10 µg/Lane Lane 5: NIH/3T3 cell lysate, 10 µg/Lane Lane 6: RAW264.7 cell lysate, 10 µg/Lane Lane 7: PC-12 cell lysate, 10 µg/Lane Lane 8: Rat spleen tissue lysate, 20 µg/Lane Predicted band size: 61 kDa Observed band size: 61 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721110) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue with Rabbit anti-PGM1 antibody (HA721110) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721110) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.