PGM1 Recombinant Rabbit Monoclonal Antibody [JE65-06]
cat.: HA721110
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | JE65-06 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 61 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human PGM1 aa 513-562/562. |
| Positive control: | A431 cell lysate, HeLa cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate, COS-1 cell lysate, Mouse spleen tissue lysate, 293T cell lysate, Hela cell lysate, RAW264.7 cell lysate, PC-12 cell lysate, Rat spleen tissue lysate, A431, PC-12. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell FC |
1:1,000-1:5,000 1:100 1:1,000 |
| Uniprot #: | SwissProt: P36871 Human | Q9D0F9 Mouse | P38652 Rat |
| Alternative names: | CDG1T Glucose phosphomutase 1 GSD14 OTTHUMP00000010519 OTTHUMP00000010520 PGM 1 PGM1 PGM1_HUMAN Phosphoglucomutase 1 Phosphoglucomutase-1 Phosphoglucomutase1 |
Images
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Fig1:
Western blot analysis of PGM1 on different lysates with Rabbit anti-PGM1 antibody (HA721110) at 1/5,000 dilution. Lane 1: A431 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: HepG2 cell lysate (20 µg/Lane) Lane 4: NIH/3T3 cell lysate (20 µg/Lane) Lane 5: COS-1 cell lysate (20 µg/Lane) Lane 6: Mouse spleen tissue lysate (30 µg/Lane) Predicted band size: 61 kDa Observed band size: 61 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721110) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of PGM1 on different lysates with Rabbit anti-PGM1 antibody (HA721110) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-PGM1 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 61 kDa Observed band size: 61 kDa Exposure time: 20 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721110) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Western blot analysis of PGM1 on different lysates with Rabbit anti-PGM1 antibody (HA721110) at 1/1,000 dilution. Lane 1: 293T cell lysate, 10 µg/Lane Lane 2: A431 cell lysate, 10 µg/Lane Lane 3: Hela cell lysate, 10 µg/Lane Lane 4: HepG2 cell lysate, 10 µg/Lane Lane 5: NIH/3T3 cell lysate, 10 µg/Lane Lane 6: RAW264.7 cell lysate, 10 µg/Lane Lane 7: PC-12 cell lysate, 10 µg/Lane Lane 8: Rat spleen tissue lysate, 20 µg/Lane Predicted band size: 61 kDa Observed band size: 61 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721110) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig4:
Immunocytochemistry analysis of A431 cells labeling PGM1 with Rabbit anti-PGM1 antibody (HA721110) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PGM1 antibody (HA721110) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig5:
Immunocytochemistry analysis of PC-12 cells labeling PGM1 with Rabbit anti-PGM1 antibody (HA721110) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PGM1 antibody (HA721110) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig6:
Flow cytometric analysis of A431 cells labeling PGM1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721110, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig7:
Flow cytometric analysis of PC-12 cells labeling PGM1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721110, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.