NOLC1 Recombinant Rabbit Monoclonal Antibody [JE65-16]
cat.: HA721113
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE65-16 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 74 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human NOLC1 aa 41-90/699. |
| Positive control: | K562 cell lysate, Hela cell lysate, HepG2 cell lysate, MCF-7 cell lysate, human kidney tissue. |
| Subcellular location: | Nucleolus, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:500 1:400 |
| Uniprot #: | SwissProt: Q14978 Human |
| Alternative names: | 140 kDa nucleolar phosphoprotein HCV NS5A trans regulated protein 13 HCV NS5A transactivated protein 13 HCV NS5A-transactivated protein 13 Hepatitis C virus NS5A transactivated protein 13 Hepatitis C virus NS5A-transactivated protein 13 KIAA0035 NOLC 1 NOLC1 NOLC1_HUMAN NOPP 130 NOPP 140 NOPP130 Nopp140 NS5ATP13 Nucleolar 130 kDa protein Nucleolar and coiled body phosphoprotein 1 Nucleolar and coiled body phosphprotein 1 Nucleolar and coiled-body phosphoprotein 1 Nucleolar phosphoprotein p130 Nucleolar protein p130 P130 |
Images
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Fig1:
Western blot analysis of NOLC1 on different lysates with Rabbit anti-NOLC1 antibody (HA721113) at 1/500 dilution. Lane 1: K562 cell lysate Lane 2: Hela cell lysate Lane 3: HepG2 cell lysate Lane 4: MCF-7 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 74 kDa Observed band size: 120 kDa Exposure time: 30 seconds; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721113) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-NOLC1 antibody (HA721113) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721113) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.