SF3A3 Recombinant Rabbit Monoclonal Antibody [JE64-15]
cat.: HA721114
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE64-15 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 59 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human SF3A3 aa 1-50/501. |
| Positive control: | MCF-7 cell lysate, 293T cell lysate, Hela cell lysate, rat testis tissue lysates, rat brain tissue lysates, rat lung tissue lysate, mouse cerebellum tissue lysate, mouse stomach tissue lysate, rat kidney tissue, human testis tissue, human colon carcinoma tissue, human prostate tissue, mouse small intestine tissue. |
| Subcellular location: | Nucleus speckle, Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:500 1:400 |
| Uniprot #: | SwissProt: Q12874 Human | Q9D554 Mouse Entrez Gene: 313583 Rat |
| Alternative names: | SF3A3 Pre mRNA splicing factor SF3a (60kD) Pre mRNA splicing factor SF3a PRP 9 PRP9 PRPF 9 PRPF9 SAP 61 SAP61 SF3a60 Spliceosome associated protein 61 Splicing factor 3a subunit 3 60kDa Splicing factor 3a subunit 3 |
Images
|
Fig1:
Western blot analysis of SF3A3 on different lysates with Rabbit anti-SF3A3 antibody (HA721114) at 1/500 dilution. Lane 1: MCF-7 cell lysate Lane 2: 293T cell lysate Lane 3: Hela cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 59 kDa Observed band size: 59 kDa Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721114) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of SF3A3 on rat testis tissue lysates with Rabbit anti-SF3A3 antibody (HA721114) at 1/500 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 59 kDa Observed band size: 59 kDa Exposure time: 30 seconds; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721114) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Western blot analysis of SF3A3 on rat brain tissue lysates with Rabbit anti-SF3A3 antibody (HA721114) at 1/500 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 59 kDa Observed band size: 59 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721114) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig4:
Western blot analysis of SF3A3 on different lysates with Rabbit anti-SF3A3 antibody (HA721114) at 1/500 dilution. Lane 1: Rat lung tissue lysate Lane 2: Mouse cerebellum tissue lysate Lane 3: Mouse stomach tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 59 kDa Observed band size: 59 kDa Exposure time: 30 seconds; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721114) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-SF3A3 antibody (HA721114) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721114) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig6:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-SF3A3 antibody (HA721114) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721114) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig7:
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-SF3A3 antibody (HA721114) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721114) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig8:
Immunohistochemical analysis of paraffin-embedded human prostate tissue with Rabbit anti-SF3A3 antibody (HA721114) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721114) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig9:
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue with Rabbit anti-SF3A3 antibody (HA721114) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721114) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.