MCM6 Recombinant Rabbit Monoclonal Antibody [JE65-68]
cat.: HA721117
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P
Clonality: Monoclonal
Clone number: JE65-68
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 93 kDa
Isotype: IgG
Immunogen: Recombinant protein within human MCM6 aa 572-821/821.
Positive control: Hela cell lysate, Jurkat cell lysate, MCF-7 cell lysate, RAW264.7 cell lysate, SiHa, rat skin tissue, human lymph nodes tissue, human testis tissue, mouse stomach tissue.
Subcellular location: Nucleus, Chromosome.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P

1:500
1:200
1:400
Uniprot #: SwissProt: Q14566 Human | P97311 Mouse | Q62724 Rat
Alternative names: DNA replication licensing factor MCM6 MCG40308 Mcm6 MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe) MCM6 minichromosome maintenance deficient 6 MCM6_HUMAN Minichromosome maintenance complex component 6 Minichromosome maintenance deficient (mis5 S. pombe) 6 Minichromosome maintenance deficient 6 homolog Minichromosome maintenance protein 6 Mis5 MIS5 homolog p105MCM
Images
HA721117_1.jpg Fig1: Western blot analysis of MCM6 on different lysates with Rabbit anti-MCM6 antibody (HA721117) at 1/500 dilution.

Lane 1: Hela cell lysate
Lane 2: Jurkat cell lysate
Lane 3: MCF-7 cell lysate
Lane 4: RAW264.7 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 93 kDa
Observed band size: 93 kDa

Exposure time: 2 minutes;

8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721117) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
HA721117_2.jpg Fig2: Immunocytochemistry analysis of SiHa cells labeling MCM6 with Rabbit anti-MCM6 antibody (HA721117) at 1/200 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-MCM6 antibody (HA721117) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) were used as the secondary antibody at 1/1,000 dilution.
HA721117_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat skin tissue with Rabbit anti-MCM6 antibody (HA721117) at 1/400 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721117) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721117_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-MCM6 antibody (HA721117) at 1/400 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721117) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721117_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-MCM6 antibody (HA721117) at 1/400 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721117) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721117_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue with Rabbit anti-MCM6 antibody (HA721117) at 1/400 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721117) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.