PCPTP1/PTPRQ Recombinant Rabbit Monoclonal Antibody [JE64-84]
cat.: HA721118
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE64-84 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 74 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human PCPTP1 aa 1-50/657. |
| Positive control: | Rat brain tissue lysates, human stomach tissue. |
| Subcellular location: | Cell membrane; Perinuclear region. |
| Recommended Dilutions:
WB IHC-P |
1:500 1:100 |
| Uniprot #: | SwissProt: Q15256 Human | O08617 Rat |
| Alternative names: | Ch-1PTPase Ch1 PTPase DKFZp781C1038 EC PTP ECPTP FLJ34328 MGC131968 MGC148170 NC PTPCOM1 NC-PTPCOM1 Protein tyrosine phosphatase Cr1PTPase precursor Protein tyrosine phosphatase NC PTPCOM1 Protein tyrosine phosphatase PCPTP1 Protein tyrosine phosphatase receptor type R Protein-tyrosine phosphatase PCPTP1 PTP SL PTPBR7 PTPRQ PTPRR PTPRR_HUMAN PTPSL R-PTP-R Receptor type tyrosine protein phosphatase R Receptor-type tyrosine-protein phosphatase R |
Images
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Fig1:
Western blot analysis of PCPTP1/PTPRQ on Rat brain tissue lysates with Rabbit anti-PCPTP1/PTPRQ antibody (HA721118) at 1/500 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 74 kDa Observed band size: 74 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721118) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-PCPTP1/PTPRQ antibody (HA721118) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721118) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.