MYO1B Recombinant Rabbit Monoclonal Antibody [JE65-01]
cat.: HA721119
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE65-01 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 132 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human MYO1B aa 916-1,136/1,136. |
| Positive control: | C6 cell lysate, Mouse heart tissue lysate, Rat heart tissue lysate, A431 cell lysate, NIH/3T3 cell lysate, human lymph nodes tissue, human testis tissue, human colon carcinoma tissue, mouse liver tissue, rat liver tissue. |
| Subcellular location: | Actin cytoskeleton, actin filament, myosin complex, early endosome, endosome membrane, extracellular exosome, plasma membrane, apical part of cell, brush border, cell periphery, cytoplasm, filopodium, microvillus, perinuclear region of cytoplasm, vesicle |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:5,000 1:200-1:500 |
| Uniprot #: | SwissProt: O43795 Human | P46735 Mouse | Q05096 Rat |
| Alternative names: | MMI alpha MMI-alpha MMIa MYH 1c MYH-1c Myo1b MYO1B variant protein MYO1B_HUMAN Myosin I alpha myosin IB Myosin-Ib myr1 OTTHUMP00000165861 Unconventional myosin Ib |
Images
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Fig1:
Western blot analysis of MYO1B on different lysates with Rabbit anti-MYO1B antibody (HA721119) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-MYO1B KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 132 kDa Observed band size: 132 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721119) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of MYO1B on different lysates with Rabbit anti-MYO1B antibody (HA721119) at 1/5,000 dilution. Lane 1: C6 cell lysate Lane 2: Mouse heart tissue lysate Lane 3: Rat heart tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 132 kDa Observed band size: 132 kDa Exposure time: Lane 1: 10 seconds; Lane 2-3: 2 minutes 24 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721119) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Western blot analysis of MYO1B on different lysates with Rabbit anti-MYO1B antibody (HA721119) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: NIH/3T3 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 132 kDa Observed band size: 132 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721119) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-MYO1B antibody (HA721119) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721119) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-MYO1B antibody (HA721119) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721119) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-MYO1B antibody (HA721119) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721119) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-MYO1B antibody (HA721119) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721119) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig8:
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-MYO1B antibody (HA721119) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721119) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.