PIN4 Recombinant Rabbit Monoclonal Antibody [JE64-05]
cat.: HA721121
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE64-05 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 14 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human PIN4 aa 82-131/131. |
| Positive control: | 293T cell lysates, rat large intestine tissue, human testis tissue, human colon carcinoma tissue, mouse esophagus tissue, mouse stomach tissue. |
| Subcellular location: | Nucleolus, Cytoplasm, spindle; Mitochondrion, Mitochondrion matrix. |
| Recommended Dilutions:
WB IHC-P |
1:500 1:2,000 |
| Uniprot #: | SwissProt: Q9Y237 Human | Q9CWW6 Mouse Entrez Gene: 684441 Rat |
| Alternative names: | EPVH Eukaryotic parvulin homolog hEPVH hPar14 hPar17 MGC138486 OTTHUMP00000023527 OTTHUMP00000217483 OTTHUMP00000217485 Par14 Par17 Parvulin 14 Parvulin-14 Parvulin-17 Peptidyl prolyl cis trans isomerase NIMA interacting 4 Peptidyl prolyl cis/trans isomerase EPVH Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4 Peptidyl-prolyl cis-trans isomerase PIN4 Peptidyl-prolyl cis/trans isomerase EPVH PIN4 PIN4_HUMAN PPIase Pin4 Protein (peptidylprolyl cis/trans isomerase) NIMA interacting, 4 (parvulin) Rotamase Pin4 |
Images
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Fig1:
Western blot analysis of PIN4 on 293T cell lysates with Rabbit anti-PIN4 antibody (HA721121) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 14 kDa Observed band size: 14 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721121) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded rat large intestine tissue with Rabbit anti-PIN4 antibody (HA721121) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721121) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-PIN4 antibody (HA721121) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721121) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-PIN4 antibody (HA721121) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721121) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded mouse esophagus tissue with Rabbit anti-PIN4 antibody (HA721121) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721121) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig6:
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue with Rabbit anti-PIN4 antibody (HA721121) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721121) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.