Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB, IHC-P, IF-Cell |
Clonality: | Monoclonal |
Clone number: | JE64-05 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 14 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within human PIN4 aa 82-131/131. |
Positive control: | HEK-293 cell lysate, RAW264.7 cell lysate, C6 cell lysate, Mouse stomach tissue lysate, Mouse liver tissue lysate, Rat liver tissue lysate, HEK-293, RAW264.7, rat large intestine tissue, human testis tissue, human colon carcinoma tissue, mouse esophagus tissue, mouse stomach tissue. |
Subcellular location: | Nucleolus, Cytoplasm, spindle; Mitochondrion, Mitochondrion matrix. |
Recommended Dilutions:
WB IHC-P IF-Cell |
1:2,000 1:2,000 1:100 |
Uniprot #: | SwissProt: Q9Y237 Human | Q9CWW6 Mouse Entrez Gene: 684441 Rat |
Alternative names: | EPVH Eukaryotic parvulin homolog hEPVH hPar14 hPar17 MGC138486 OTTHUMP00000023527 OTTHUMP00000217483 OTTHUMP00000217485 Par14 Par17 Parvulin 14 Parvulin-14 Parvulin-17 Peptidyl prolyl cis trans isomerase NIMA interacting 4 Peptidyl prolyl cis/trans isomerase EPVH Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4 Peptidyl-prolyl cis-trans isomerase PIN4 Peptidyl-prolyl cis/trans isomerase EPVH PIN4 PIN4_HUMAN PPIase Pin4 Protein (peptidylprolyl cis/trans isomerase) NIMA interacting, 4 (parvulin) Rotamase Pin4 |
Fig1:
Western blot analysis of PIN4 on different lysates with Rabbit anti-PIN4 antibody (HA721121) at 1/2,000 dilution. Lane 1: HEK-293 cell lysate (20 µg/Lane) Lane 2: RAW264.7 cell lysate (20 µg/Lane) Lane 3: C6 cell lysate (20 µg/Lane) Lane 4: Mouse stomach tissue lysate (40 µg/Lane) Lane 5: Mouse liver tissue lysate (40 µg/Lane) Lane 6: Rat liver tissue lysate (40 µg/Lane) Predicted band size: 14 kDa Observed band size: 14 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721121) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of PIN4 on different lysates with Rabbit anti-PIN4 antibody (HA721121) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-PIN4 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 14 kDa Observed band size: 14 kDa Exposure time: 120 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721121) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Fig3:
Immunocytochemistry analysis of HEK-293 cells labeling PIN4 with Rabbit anti-PIN4 antibody (HA721121) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PIN4 antibody (HA721121) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Counterstained with Mitotracker. Nuclear DNA was labelled in blue with DAPI. |
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Fig4:
Immunocytochemistry analysis of RAW264.7 cells labeling PIN4 with Rabbit anti-PIN4 antibody (HA721121) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PIN4 antibody (HA721121) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Counterstained with Mitotracker. Nuclear DNA was labelled in blue with DAPI. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat large intestine tissue with Rabbit anti-PIN4 antibody (HA721121) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721121) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Fig6:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-PIN4 antibody (HA721121) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721121) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-PIN4 antibody (HA721121) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721121) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig8:
Immunohistochemical analysis of paraffin-embedded mouse esophagus tissue with Rabbit anti-PIN4 antibody (HA721121) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721121) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig9:
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue with Rabbit anti-PIN4 antibody (HA721121) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721121) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |