NDUFC2 Recombinant Rabbit Monoclonal Antibody [JE65-31]
cat.: HA721123
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE65-31 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 14 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human NDUFC2 aa 1-119. |
| Positive control: | 293T cell lysate, Hela cell lysate, A549 cell lysate, Human kidney tissue lysate, human kidney tissue, human liver tissue. |
| Subcellular location: | Mitochondrion inner membrane. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:20-1:1,000 |
| Uniprot #: | SwissProt: O95298 Human |
| Alternative names: | B14.5b CI-B14.5b Complex I B14.5b Complex I subunit B14.5b Complex I-B14.5b HLC-1 HLC1 Human lung cancer oncogene 1 protein NADH dehydrogenase (ubiquinone) 1, subcomplex unknown, 2, 14.5kDa NADH dehydrogenase [ubiquinone] 1 subunit C2 NADH dehydrogenase ubiquinone 1 subunit C2 NADH ubiquinone oxidoreductase subunit B14.5b NADH-ubiquinone oxidoreductase subunit B14.5b NADHDH2 NDUC2_HUMAN NDUFC2 |
Images
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Fig1:
Western blot analysis of NDUFC2 on different lysates with Rabbit anti-NDUFC2 antibody (HA721123) at 1/1,000 dilution. Lane 1: 293T cell lysate (10 µg/Lane) Lane 2: Hela cell lysate (10 µg/Lane) Lane 3: A549 cell lysate (10 µg/Lane) Lane 4: Human kidney tissue lysate (20 µg/Lane) Predicted band size: 14 kDa Observed band size: 14 kDa Exposure time: 1 minute; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721123) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-NDUFC2 antibody (HA721123) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721123) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-NDUFC2 antibody (HA721123) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721123) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.