PEX11B Recombinant Rabbit Monoclonal Antibody [JE64-51]
cat.: HA721124
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE64-51 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 28 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human PEX11B aa 101-150/259. |
| Positive control: | Human lung tissue lysate, MCF-7 cell lysate, human lung carcinoma tissue, human colon carcinoma tissue, human breast carcinoma tissue. |
| Subcellular location: | Peroxisome membrane. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:400 |
| Uniprot #: | SwissProt: O96011 Human |
| Alternative names: | OTTHUMP00000015598 Peroxin 11B Peroxin-11B peroxisomal biogenesis factor 11 beta Peroxisomal biogenesis factor 11B Peroxisomal membrane protein 11B PEX11 beta PEX11-beta PEX11B Pex11p beta PEX14B Protein PEX11 homolog beta PX11B_HUMAN |
Images
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Fig1:
Western blot analysis of PEX11B on different lysates with Rabbit anti-PEX11B antibody (HA721124) at 1/1,000 dilution. Lane 1: Human lung tissue lysate, 20 µg/Lane Lane 2: MCF-7 cell lysate, 10 µg/Lane. Predicted band size: 28 kDa Observed band size: 25 kDa Exposure time: 1 minute; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721124) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of PEX11B on different lysates with Rabbit anti-PEX11B antibody (HA721124) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-PEX11B KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 28 kDa Observed band size: 28 kDa Exposure time: 40 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721124) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-PEX11B antibody (HA721124) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721124) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-PEX11B antibody (HA721124) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721124) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-PEX11B antibody (HA721124) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721124) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.