RAB8A Recombinant Rabbit Monoclonal Antibody [JE64-79]
cat.: HA721127
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IF-Cell, IP |
| Clonality: | Monoclonal |
| Clone number: | JE64-79 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 24 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human RAB8A aa 151-200/207. |
| Positive control: | Hela cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, C2C12 cell lysate, L6 cell lysate, HeLa. |
| Subcellular location: | Cell membrane, golgi apparatus, recycling endosome membrane, cytoplasm, centriole, cilium basal body, cilium axoneme, cilium, phagosome, phagosome membrane, midbody. |
| Recommended Dilutions:
WB FC IF-Cell IP |
1:500 1:500-1:1,000 1:100 1-2μg/sample |
| Uniprot #: | SwissProt: P61006 Human | P55258 Mouse | P35280 Rat |
| Alternative names: | AA409338 MEL Mel transforming oncogene (derived from cell line NK14) RAB8 homolog Mel transforming oncogene (derived from cell line NK14) Mel transforming oncogene (RAB8 homolog) Mel transforming oncogene MGC124948 Oncogene c mel Oncogene c-mel RAB8 RAB8A RAB8A member RAS oncogene family RAB8A_HUMAN Ras associated protein RAB8 Ras related protein Rab 8A Ras-related protein Rab-8A |
Images
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Fig1:
Western blot analysis of RAB8A on different lysates with Rabbit anti-RAB8A antibody (HA721127) at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: HepG2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 24 kDa Observed band size: 24 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721127) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of RAB8A on different lysates with Rabbit anti-RAB8A antibody (HA721127) at 1/500 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: RAW264.7 cell lysate Lane 3: C2C12 cell lysate Lane 4: L6 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 24 kDa Observed band size: 24 kDa Exposure time: 2 minutes 16 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721127) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunocytochemistry analysis of HeLa cells labeling RAB8A with Rabbit anti-RAB8A antibody (HA721127) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RAB8A antibody (HA721127) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig4:
Flow cytometric analysis of HeLa cells labeling RAB8A. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721127, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig5:
RAB8A was immunoprecipitated from 0.2 mg HeLa cell lysate with HA721127 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA721127 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA721127 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of HA721127 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 4 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.