COASY Recombinant Rabbit Monoclonal Antibody [JE58-56]
cat.: HA721128
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | JE58-56 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 62 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human COASY aa 510-564. |
| Positive control: | HepG2 cell lysates, HepG2. |
| Subcellular location: | Mitochondrion matrix, Cytoplasm. |
| Recommended Dilutions:
WB FC |
1:500-1:2,000 1:500-1:1,000 |
| Uniprot #: | SwissProt: Q13057 Human |
| Alternative names: | Bifunctional coenzyme A synthase Bifunctional phosphopantetheine adenylyl transferase/dephospho CoA kinase CoA synthase COASY COASY_HUMAN Coenzyme A synthase Dephospho-CoA kinase Dephospho-CoA pyrophosphorylase Dephosphocoenzyme A kinase DPCK DPCOAK NBP Nucleotide binding protein Pantetheine-phosphate adenylyltransferase Phosphopantetheine adenylyltransferase / dephosphocoenzyme A kinase pOV 2 POV-2 POV2 PPAT UKR1 |
Images
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Fig1:
Western blot analysis of COASY on HepG2 cell lysates with Rabbit anti-COASY antibody (HA721128) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 60 kDa Observed band size: 60 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721128) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Flow cytometric analysis of COASY was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (HA721128, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.