COASY Recombinant Rabbit Monoclonal Antibody [JE58-56]
cat.: HA721128
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, FC, IF-Cell
Clonality: Monoclonal
Clone number: JE58-56
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 62 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human COASY aa 510-564.
Positive control: HepG2 cell lysate, HeLa cell lysate, A431 cell lysate, HepG2.
Subcellular location: Mitochondrion matrix, Cytoplasm.
Recommended Dilutions:
  WB
  FC
  IF-Cell
1:1,000-1:2,000
1:1,000
1:100
Uniprot #: SwissProt: Q13057 Human
Alternative names: Bifunctional coenzyme A synthase Bifunctional phosphopantetheine adenylyl transferase/dephospho CoA kinase CoA synthase COASY COASY_HUMAN Coenzyme A synthase Dephospho-CoA kinase Dephospho-CoA pyrophosphorylase Dephosphocoenzyme A kinase DPCK DPCOAK NBP Nucleotide binding protein Pantetheine-phosphate adenylyltransferase Phosphopantetheine adenylyltransferase / dephosphocoenzyme A kinase pOV 2 POV-2 POV2 PPAT UKR1
Images
HA721128_1.jpg Fig1: Western blot analysis of COASY on different lysates with Rabbit anti-COASY antibody (HA721128) at 1/1,000 dilution.

Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-COASY KD cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 62 kDa
Observed band size: 62 kDa

Exposure time: 120 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721128) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA721128_2.jpg Fig2: Western blot analysis of COASY on different lysates with Rabbit anti-COASY antibody (HA721128) at 1/2,000 dilution.

Lane 1: HepG2 cell lysate
Lane 2: HeLa cell lysate
Lane 3: A431 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 62 kDa
Observed band size: 62 kDa

Exposure time: 25 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721128) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA721128_3.jpg Fig3: Flow cytometric analysis of HepG2 cells labeling COASY.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA721128, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
HA721128_4.jpg Fig4: Immunocytochemistry analysis of HepG2 cells labeling COASY with Rabbit anti-COASY antibody (HA721128) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-COASY antibody (HA721128) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Counterstained with Mitotracker. Nuclear DNA was labelled in blue with DAPI.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.