Villin1 Recombinant Rabbit Monoclonal Antibody [C4]
cat.: HA721129
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | C4 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 93 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Villin1 aa 600-827. |
| Positive control: | HT-29 (Human colorectal adenocarcinoma cell) cell lysate, Caco-2 (Human colorectal adenocarcinoma cell) cell lysate, Hep G2 (Human hepatocellular carcinoma cell) cell lysate, Mouse small intestine tissue lysate, Mouse kidney tissue lysate, Rat small intestine tissue lysate, human kidney tissue, human small intestine tissue, mouse small intestine tissue, rat small intestine tissue. |
| Subcellular location: | Cytoskeleton, lamellipodium, ruffle, microvillus, filopodium tip, filopodium. |
| Recommended Dilutions:
WB IHC-P |
1:10,000 1:3,500-1:20,000 |
| Uniprot #: | SwissProt: P09327 Human |
| Alternative names: | D2S1471 OTTHUMP00000164145 VIL VIL1 VILI_HUMAN Villin 1 Villin-1 Villin1 |
Images
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Fig1:
Western blot analysis of Villin1 on different lysates with Rabbit anti-Villin1 antibody (HA721129) at 1/10,000 dilution. Lane 1: HT-29 (Human colorectal adenocarcinoma cell) cell lysate Lane 2: Caco-2 (Human colorectal adenocarcinoma cell) cell lysate Lane 3: Hep G2 (Human hepatocellular carcinoma cell) cell lysate Lane 4: Mouse small intestine tissue lysate Lane 5: Mouse kidney tissue lysate Lane 6: Rat small intestine tissue lysate Cell Lysates/proteins at 15 µg/Lane, Tissue Lysates/proteins at 30 µg/Lane. Exposure time: 17 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA721129, 1/10,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 93 kDa Observed band size: 93 kDa |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Villin1 antibody (HA721129) at 1/3,500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721129) at 1/3,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-Villin1 antibody (HA721129) at 1/3,500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721129) at 1/3,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Application: Immunohistochemistry (IHC-P) Species: Mouse Tissue: Small intestine Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA721129, 1/20,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig5:
Application: Immunohistochemistry (IHC-P) Species: Rat Tissue: Small intestine Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA721129, 1/20,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.