HER3 Recombinant Rabbit Monoclonal Antibody [R4]
cat.: HA721135
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | FC |
| Clonality: | Monoclonal |
| Clone number: | R4 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 148 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human HER3 Extracellular domain. |
| Positive control: | MCF-7, SK-Br-3. |
| Subcellular location: | Cell membrane; Secreted. |
| Recommended Dilutions:
FC |
1:100-1:1,000 |
| Uniprot #: | SwissProt: P21860 Human |
| Alternative names: | c erbB 3 c erbB3 Erb b2 receptor tyrosine kinase 3 ErbB 3 ERBB3 ERBB3 protein erbB3 S ERBB3_HUMAN Glial growth factor receptor HER 3 HER3 Human epidermal growth factor receptor 3 LCCS2 MDA BF 1 MGC88033 p180 ErbB3 p45 sErbB3 p85 sErbB3 proto-oncogene-like protein c ErbB 3 proto-oncogene-like protein c ErbB3 Proto-oncogene-like protein c-ErbB-3 Receptor tyrosine protein kinase erbB 3 Receptor tyrosine protein kinase erbB3 Receptor tyrosine-protein kinase erbB-3 Tyrosine kinase type cell surface receptor HER3 Tyrosine kinase-type cell surface receptor HER3 v erb b2 avian erythroblastic leukemia viral oncogene homolog 3 v erb b2 erythroblastic leukemia viral oncogene homolog 3 (avian) v erb b2 erythroblastic leukemia viral oncogene homolog 3 |
Images
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Fig1:
Flow cytometric analysis of MCF-7 cells labeling HER3. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA721135, 0.1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
|
Fig2:
Flow cytometric analysis of SK-Br-3 cells labeling HER3. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA721135, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.