CD5 Recombinant Rabbit Monoclonal Antibody [PD00-01]
cat.: HA721137
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IHC-P, IF-Tissue, WB |
| Clonality: | Monoclonal |
| Clone number: | PD00-01 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 55 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human CD5 aa 403 – 495 (intracellular). |
| Positive control: | Human lymph nodes tissue, human spleen tissue, Jurkat cell lysates. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
IHC-P IF-Tissue WB |
1:1,500 1:200 1:5,000 |
| Uniprot #: | SwissProt: P06127 Human |
| Alternative names: | CD 5 CD5 CD5 antigen (p56 62) CD5 antigen CD5 molecule CD5_HUMAN LEU1 LyA Lymphocyte antigen T1/Leu-1 OTTHUMP00000236973 T-cell surface glycoprotein CD5 T1 |
Images
|
Fig1:
Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-CD5 antibody (HA721137) at 1/1,500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721137) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD5 antibody (HA721137) at 1/1,500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721137) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Application: IF-Tissue Species: Human Site: spleen Sample: Paraffin-embedded section Antibody concentration: 1/200 |
|
Fig4:
Western blot analysis of CD5 on Jurkat cell lysates with Rabbit anti-CD5 antibody (HA721137) at 1/5,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 55 kDa Observed band size: 67 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721137) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.