CD23 Recombinant Rabbit Monoclonal Antibody [PD00-03]
cat.: HA721139
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IHC-P, mIHC, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | PD00-03 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 36 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CD23 aa 48 – 321 (Extracellular). |
| Positive control: | Human tonsil tissue, human lymph nodes tissue, human spleen tissue. |
| Subcellular location: | Cell membrane, Secreted. |
| Recommended Dilutions:
IHC-P mIHC IF-Tissue |
1:200-1:2,000 1:800 1:200 |
| Uniprot #: | SwissProt: P06734 Human |
| Alternative names: | Blast 2 BLAST-2 Blast2 C type lectin domain family 4 member J C-type lectin domain family 4 C-type lectin domain family 4 member J C-type lectin domain family 4, member J CD 23 CD 23A CD23 CD23 antigen CD23A CLEC 4J CLEC4J Fc epsilon receptor II Fc epsilon RII Fc fragment of IgE Fc fragment of IgE low affinity II receptor for Fc fragment of IgE receptor II Fc fragment of IgE, low affinity II, receptor for (CD23) Fc of IgE Fc of IgE, low affinity II, receptor for (CD23) Fc receptor IgE low affinity II alpha polypeptide Fc receptor, IgE, low affinity II, alpha polypeptide, isoform CRA_a Fc-epsilon-RII FCE 2 FCE2 FCER 2 Fcer2 FCER2_HUMAN FCER2A FceRII IgE binding factor IgE receptor lymphocyte IgE-binding factor IGEBF Immunoglobulin E binding factor Immunoglobulin E receptor Immunoglobulin E receptor, low affinity II Immunoglobulin E-binding factor Immunoglobulin epsilon chain LEUKOCYTE ANTIGEN CD23 Low Af...... |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD23 antibody (HA721139) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721139) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-CD23 antibody (HA721139) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721139) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD23 antibody (HA721139) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721139) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Fluorescence multiplex immunohistochemical analysis of Human tonsil (Formalin/PFA-fixed paraffin-embedded sections). Panel A: the merged image of anti-CD68 (HA601115, Red), anti-CD38 (HA721268, Green), anti-CD23 (HA721139, White), anti-CD11C (ET1606-19, Cyan), anti-CD45 (ET7111-03, Magenta) and anti-CD20 (HA721138, Yellow) on tonsil. Panel B: anti-CD68 stained on Macrophage. Panel C: anti-CD38 stained on lymphocyte subsets. Panel D: anti-CD11C stained on dendritic cells. Panel E: CD45 stained on lymphocytes. Panel F: anti-CD20 stained on B cells. Panel G: anti-CD23 stained on follicular dendritic cells. HRP Conjugated UltraPolymer Goat Polyclonal Antibody HA1119/HA1120 was used as a secondary antibody. The immunostaining was performed with the Sequential Immuno-staining Kit (IRISKit™MH010101, www.luminiris.cn). The section was incubated in six rounds of staining: in the order of HA601115 (1/2,000 dilution), HA721268 (1/1,000 dilution), ET1606-19 (1/1,000 dilution), ET7111-03 (1/500 dilution), HA721138 (1/2,000 dilution) and HA721139 (1/800 dilution) for 20 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner. |
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Fig5:
Application: IF-Tissue Species: Human Site: tonsil Sample: Paraffin-embedded section Antibody concentration: 1/200 |
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Fig6:
Application: IF-Tissue Species: Human Site: lymph nodes Sample: Paraffin-embedded section Antibody concentration: 1/200 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.