TGF beta 1 Recombinant Rabbit Monoclonal Antibody [PD00-17]
cat.: HA721143
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | PD00-17 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 44 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide (KLH-coupled) corresponding to Human TGF-Beta 1 C-terminal. |
| Positive control: | K-562 cell lysate, Raji cell lysate, Saos-2 cell lysate, 786-0 cell lysate, COS-1 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, L-929 cell lysate, C6 cell lysate, Mouse spleen tissue lysate, Rat spleen tissue lysate, rat spleen tissue, mouse spleen tissue. |
| Subcellular location: | Extracellular matrix, Secreted. |
| Recommended Dilutions:
WB IHC-P IF-Tissue |
1:2,000-1:5,000 1:200 1:200 |
| Uniprot #: | SwissProt: P01137 Human | P04202 Mouse | P17246 Rat |
| Alternative names: | Cartilage-inducing factor CED Differentiation inhibiting factor DPD1 LAP Latency-associated peptide Prepro transforming growth factor beta 1 TGF beta 1 TGF beta TGF beta 1 protein TGF-beta 1 protein TGF-beta-1 TGF-beta-5 TGF-beta1 TGFB Tgfb-1 tgfb1 TGFB1_HUMAN TGFbeta TGFbeta1 Transforming Growth Factor b1 Transforming Growth Factor beta 1 Transforming growth factor beta 1a transforming growth factor beta-1 transforming growth factor, beta 1 Transforming Growth Factor-ß1 |
Images
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Fig1:
Western blot analysis of TGF beta 1 on different lysates with Rabbit anti-TGF beta 1 antibody (HA721143) at 1/5,000 dilution. Lane 1: K-562 cell lysate Lane 2: Raji cell lysate Lane 3: Saos-2 cell lysate Lane 4: 786-0 cell lysate Lane 5: COS-1 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: RAW264.7 cell lysate Lane 8: L-929 cell lysate Lane 9: C6 cell lysate Lane 10: Mouse spleen tissue lysate Lane 11: Mouse skeletal muscle tissue lysate (negative) Lane 12: Rat spleen tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 44 kDa Observed band size: 48/13 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721143) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-TGF beta 1 antibody (HA721143) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721143) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-TGF beta 1 antibody (HA721143) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721143) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.