Gasdermin D (N terminal) Recombinant Rabbit Monoclonal Antibody [PD00-18]
cat.: HA721144
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IF-Cell
Clonality: Monoclonal
Clone number: PD00-18
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 53/30 kDa
Isotype: IgG
Immunogen: Recombinant protein within Gasdermin D full length protein.
Positive control: SiHa cell lysate, Jurkat cell lysate, THP-1 cell lysate, PC-3M cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, L6 cell lysate, THP-1 cell lysate treated with PMA for 17.5 hours then treated with LPS for 6 hours, mouse intestine tissue.
Subcellular location: Cell membrane, Secreted.
Recommended Dilutions:
  WB
  IHC-P
  IF-Cell

1:500-1:2,000
1:1,000
1:100
Uniprot #: SwissProt: P57764 Human | Q9D8T2 Mouse
Entrez Gene: 513939 Rat
Alternative names: 1810036L03Rik DF 5L DF5L DFNA 5L DFNA5L FKSG 10 FKSG10 FLJ12150 Gasdermin Gasdermin D Gasdermin domain containing 1 Gasdermin domain containing protein 1 Gasdermin domain-containing protein 1 Gasdermin-D GasderminD GSDMD GSDMD_HUMAN GSDMDC 1 GSDMDC1
Images
HA721144_1.jpg Fig1: Western blot analysis of Gasdermin D (N terminal) on different lysates with Rabbit anti-Gasdermin D (N terminal) antibody (HA721144) at 1/1,000 dilution.

Lane 1: SiHa cell lysate
Lane 2: Jurkat cell lysate
Lane 3: THP-1 cell lysate
Lane 4: PC-3M cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: PC-12 cell lysate
Lane 7: L6 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 53/30 kDa
Observed band size: 53/30 kDa

Exposure time: 43 seconds; ECL: K1801;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721144) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA721144_2.jpg Fig2: Western blot analysis of Gasdermin D (N terminal) on different lysates with Rabbit anti-Gasdermin D (N terminal) antibody (HA721144) at 1/500 dilution.

Lane 1: THP-1 cell lysate
Lane 2: THP-1 cell lysate treated with PMA for 17.5 hours then treated with LPS for 6 hours

Lysates/proteins at 10 µg/Lane.

Predicted band size: 53 kDa
Observed band size: 53/35 kDa

Exposure time: 30 seconds;
10% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721144) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
HA721144_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse intestine tissue with Rabbit anti-Gasdermin D (N terminal) antibody (HA721144) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721144) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.