Gasdermin D (N terminal) Recombinant Rabbit Monoclonal Antibody [PD00-18]
cat.: HA721144
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | PD00-18 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 53/30 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Gasdermin D full length protein. |
| Positive control: | SiHa cell lysate, Jurkat cell lysate, THP-1 cell lysate, PC-3M cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, L6 cell lysate, THP-1 cell lysate treated with PMA for 17.5 hours then treated with LPS for 6 hours, mouse intestine tissue. |
| Subcellular location: | Cell membrane, Secreted. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:500-1:2,000 1:1,000 1:100 |
| Uniprot #: | SwissProt: P57764 Human | Q9D8T2 Mouse Entrez Gene: 513939 Rat |
| Alternative names: | 1810036L03Rik DF 5L DF5L DFNA 5L DFNA5L FKSG 10 FKSG10 FLJ12150 Gasdermin Gasdermin D Gasdermin domain containing 1 Gasdermin domain containing protein 1 Gasdermin domain-containing protein 1 Gasdermin-D GasderminD GSDMD GSDMD_HUMAN GSDMDC 1 GSDMDC1 |
Images
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Fig1:
Western blot analysis of Gasdermin D (N terminal) on different lysates with Rabbit anti-Gasdermin D (N terminal) antibody (HA721144) at 1/1,000 dilution. Lane 1: SiHa cell lysate Lane 2: Jurkat cell lysate Lane 3: THP-1 cell lysate Lane 4: PC-3M cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lane 7: L6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 53/30 kDa Observed band size: 53/30 kDa Exposure time: 43 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721144) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Gasdermin D (N terminal) on different lysates with Rabbit anti-Gasdermin D (N terminal) antibody (HA721144) at 1/500 dilution. Lane 1: THP-1 cell lysate Lane 2: THP-1 cell lysate treated with PMA for 17.5 hours then treated with LPS for 6 hours Lysates/proteins at 10 µg/Lane. Predicted band size: 53 kDa Observed band size: 53/35 kDa Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721144) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse intestine tissue with Rabbit anti-Gasdermin D (N terminal) antibody (HA721144) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721144) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.