Sudan I Recombinant Rabbit Monoclonal Antibody [Huam029]
cat.: HA721145
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Species independent
Applications: ELISA
Clonality: Monoclonal
Clone number: Huam029
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4).
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 248 Da
Isotype: IgG
Immunogen: Sudan I-OVA.
Positive control: Sudan I
Recommended Dilutions:
  ELISA

1:250-1:16,000
Images
HA721145_1.jpg Fig1: Indirect ELISA analysis of Sudan I was performed by coating wells of a 96-well plate with 50 µl per well of Sudan I-OVA diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 1%BSA blocking buffer, and incubated with 100 µl per well of Sudan I monoclonal antibody starting at a concentration of 20 µg/mL and serially diluting it to a concentration of 1.28 ng/mL for 1 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-Rabbit IgG secondary antibody at a dilution of 1:15,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
HA721145_2.jpg Fig2: Competitive ELISA analysis of Sudan I was performed by coating wells of a 96-well plate with 50 µl per well of Sudan I-OVA diluted in carbonate/bicarbonate buffer, at a concentration of 0.1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 1%BSA blocking buffer, and incubated with 100 µl per well of Sudan I monoclonal antibody at concentration of 0.5 µg/mL with serial diluted Sudan I starting from a concentration of 10ug/ml for 1 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-Rabbit IgG secondary antibody at a dilution of 1:15,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.