Arginase-1 Recombinant Rabbit Monoclonal Antibody [PD00-05]
cat.: HA721147
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PD00-05 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 35 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Liver Arginase (C terminal) |
| Positive control: | Human liver tissue lysates, human liver tissue. |
| Subcellular location: | Cytoplasm, Cytoplasmic granule. |
| Recommended Dilutions:
WB IHC-P |
1:500 1:1,000-1:5,000 |
| Uniprot #: | SwissProt: P05089 Human |
| Alternative names: | A I Al ARG 1 arg1 ARGI1_HUMAN Arginase 1 Arginase liver Arginase type I Arginase, liver Arginase-1 Arginase1 Liver type arginase Liver-type arginase Type I arginase |
Images
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Fig1:
Western blot analysis of Arginase-1 on human liver tissue lysates with Rabbit anti-Arginase-1 antibody (HA721147) at 1/500 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 35 kDa Observed band size: 39 kDa Exposure time: 1 minute; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721147) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Arginase-1 antibody (HA721147) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721147) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.