Liver Arginase Recombinant Rabbit Monoclonal Antibody [PD00-05]
cat.: HA721147
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | PD00-05 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 35 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Liver Arginase (C terminal) |
| Positive control: | Human liver tissue lysates, human liver tissue. |
| Subcellular location: | Cytoplasm, Cytoplasmic granule. |
| Recommended Dilutions:
WB IHC-P IF-Tissue |
1:2,000 1:1,000-1:5,000 1:500-1:1,000 |
| Uniprot #: | SwissProt: P05089 Human |
| Alternative names: | A I Al ARG 1 arg1 ARGI1_HUMAN Arginase 1 Arginase liver Arginase type I Arginase, liver Arginase-1 Arginase1 Liver type arginase Liver-type arginase Type I arginase |
Images
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Fig1:
Western blot analysis of Liver Arginase on human liver tissue lysates with Rabbit anti-Liver Arginase antibody (HA721147) at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 35 kDa Observed band size: 37 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721147) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Liver Arginase antibody (HA721147) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721147) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Application: IF-Tissue Species: Human Site: liver Sample: Paraffin-embedded section Antibody concentration: 1/500 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.