Factor XIIIa Recombinant Rabbit Monoclonal Antibody [PD00-08]
cat.: HA721148
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | PD00-08 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 83 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Factor XIIIa C terminal. |
| Positive control: | Human placenta tissue lysates, human lymph nodes tissue, human placenta tissue. |
| Subcellular location: | Secreted, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P IF-Tissue |
1:500 1:1,000-1:2,000 1:1,000 |
| Uniprot #: | SwissProt: P00488 Human |
| Alternative names: | bA525O21.1 (coagulation factor XIII, A1 polypeptide) Coagulation factor XIII A chain Coagulation factor XIII A1 polypeptide Coagulation factor XIII A1 subunit Coagulation factor XIII, A polypeptide Coagulation factor XIIIa F13A F13A_HUMAN F13a1 Factor XIIIA Fibrin stabilizing factor, A subunit Fibrinoligase FSF, A subunit Protein glutamine gamma glutamyltransferase A chain Protein-glutamine gamma-glutamyltransferase A chain TGase Transglutaminase A chain Transglutaminase, plasma Transglutaminase. plasma |
Images
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Fig1:
Western blot analysis of Factor XIIIa on human placenta tissue lysates with Rabbit anti-Factor XIIIa antibody (HA721148) at 1/500 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 83 kDa Observed band size: 83 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721148) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-Factor XIIIa antibody (HA721148) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721148) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-Factor XIIIa antibody (HA721148) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721148) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Application: IF-Tissue Species: Human Site: lymph nodes Sample: Paraffin-embedded section Antibody concentration: 1/1,000 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.