CD16 Recombinant Rabbit Monoclonal Antibody [PD00-12]
cat.: HA721149
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: IHC-P, IF-Tissue, IF-Cell, mIHC
Clonality: Monoclonal
Clone number: PD00-12
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 29 kDa
Isotype: IgG
Immunogen: Recombinant protein within human CD16 aa 17-208 (Extracellular).
Positive control: Human spleen tissue, human liver carcinoma tissue, human muscle tissue, 293T, Jurkat.
Subcellular location: Cell membrane, Secreted.
Recommended Dilutions:
  IHC-P
  IF-Tissue
  IF-Cell
  mIHC
1:1,000
1:200
1:100
1:1,000
Uniprot #: SwissProt: P08637 Human
Alternative names: CD 16 CD 16a CD16 CD16a CD16a antigen CD16B CD16b antigen Fc fragment of IgG Fc fragment of IgG low affinity IIIa receptor (CD16) Fc fragment of IgG low affinity IIIa receptor Fc fragment of IgG receptor IIIa Fc fragment of IgG, low affinity III, receptor (CD16) Fc fragment of IgG, low affinity III, receptor for (CD16) Fc fragment of IgG, low affinity IIIa, receptor (CD16) Fc fragment of IgG, low affinity IIIa, receptor (CD16a) Fc fragment of IgG, low affinity IIIa, receptor for Fc fragment of IgG, low affinity IIIb, receptor (CD16b) Fc fragment of IgG, low affinity IIIb, receptor for (CD16) Fc gamma R3 Fc gamma receptor III 2 (CD 16) Fc gamma receptor III A Fc gamma receptor IIIA Fc gamma receptor IIIb (CD 16) Fc gamma RIII alpha Fc gamma RIII Fc gamma RIII beta Fc gamma RIIIa Fc gamma RIIIb Fc of IgG Fc-gamma receptor III2 (CD 16) Fc-gamma receptor III2 (CD16) Fc-gamma receptor IIIb (CD16) Fc-gamma RIII Fc-gamma RIII-a......
Images
HA721149_1.jpg Fig1: Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD16 antibody (HA721149) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721149) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721149_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Rabbit anti-CD16 antibody (HA721149) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721149) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721149_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human muscle tissue with Rabbit anti-CD16 antibody (HA721149) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721149) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721149_4.jpg Fig4: Immunofluorescence analysis of paraffin-embedded human spleen tissue labeling CD16 with Rabbit anti-CD16 antibody (HA721149) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721149, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
HA721149_5.jpg Fig5: Immunofluorescence analysis of paraffin-embedded human colon cancer tissue labeling CD16 with Rabbit anti-CD16 antibody (HA721149) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721149, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
HA721149_6.jpg Fig6: Immunocytochemistry analysis of 293T cells labeling CD16 with Rabbit anti-CD16 antibody (HA721149) at 1/100 dilution.

Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD16 antibody (HA721149) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
HA721149_7.jpg Fig7: Immunocytochemistry analysis of Jurkat cells labeling CD16 with Rabbit anti-CD16 antibody (HA721149) at 1/100 dilution.

Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD16 antibody (HA721149) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
HA721149_8.jpg Fig8: mIHC analysis of human tonsils tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CD16 antibody (HA721149) at 1/1,000 dilution. The immunostaining was performed with the IRISKit® HyperView mTSA Kit (MH900206). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.