CD99 Recombinant Rabbit Monoclonal Antibody [PD00-13]
cat.: HA721150
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PD00-13 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 19 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human CD99 aa 148-185 (Cytoplasmic). |
| Positive control: | Human esophagus tissue, human stomach carcinoma tissue, human ovary granulosa tumor tissue. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
IHC-P |
1:1,000-1:2,000 |
| Uniprot #: | SwissProt: P14209 Human |
| Alternative names: | 12E7 Antigen identified by monoclonal 12E7, Y homolog Antigen identified by monoclonal antibodies 12E7, F21 and O13 CD99 CD99 antigen CD99 molecule CD99_HUMAN Cell surface antigen 12E7 Cell surface antigen HBA 71 Cell surface antigen O13 E2 antigen HBA71 MIC 2X MIC 2Y MIC2 (monoclonal 12E7) MIC2 MIC2X MIC2Y MSK5X Protein MIC2 Surface antigen MIC2 T cell surface glycoprotein E2 T-cell surface glycoprotein E2 |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded human esophagus tissue with Rabbit anti-CD99 antibody (HA721150) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721150) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue with Rabbit anti-CD99 antibody (HA721150) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721150) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human ovary granulosa tumor tissue with Rabbit anti-CD99 antibody (HA721150) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721150) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.