Placental alkaline phosphatase (PLAP) Recombinant Rabbit Monoclonal Antibody [PD00-19]
cat.: HA721151
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PD00-19 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 58 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant fragment within Human Placental alkaline phosphatase (PLAP) aa 150-300. |
| Positive control: | Human placenta tissue lysates. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P |
1:500 1:5,000-1:10,000 |
| Uniprot #: | SwissProt: P05187 Human |
| Alternative names: | Alkaline phosphatase Alkaline phosphatase placental Alkaline phosphatase placental type Alkaline phosphatase Regan isozyme ALP Alp1 ALPP FLJ61142 Germ-cell alkaline phosphatase nagao isozyme OTTHUMP00000164354 PALP Placental alkaline phosphatase 1 placental heat-stable alkaline phosphatase placental type PLAP PLAP-1 PLAP1 PPB1_HUMAN |
Images
|
Fig1:
Western blot analysis of Placental alkaline phosphatase (PLAP) on human placenta tissue lysates with Rabbit anti-Placental alkaline phosphatase (PLAP) antibody (HA721151) at 1/500 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 58 kDa Observed band size: 70 kDa Exposure time: 30 seconds; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721151) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Application: Immunohistochemistry (IHC-P) Species: Human Tissue: Placenta Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA721151, 1/10,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.