m6A Recombinant Rabbit Monoclonal Antibody [1G1]
cat.: HA721152
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | Dot Blot, ELISA |
| Clonality: | Monoclonal |
| Clone number: | 1G1 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | m6A Small Molecule conjugated to OVA. |
| Recommended Dilutions:
Dot Blot ELISA |
1:500-1:2,000 1:1,000-1:2,000 |
| Alternative names: | m6A N6-methyladenosine |
Images
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Fig1:
Dot blot analysis of N6-methyladenosine (m6A) using HA721152. Loading: total RNA extracted from HeLa Blocking Buffer: 5% NFDM/TBST; Incubation time and temperature: 1 hours at room temperature; Primary Antibody: HA721152 Dilution: 2 ug/ml, 1 ug/ml, 0.5 ug/ml, 0.25 ug/ml, 0.125 ug/ml, 0.0625 ug/ml Dilution Buffer: Primary Antibody Dilution Buffer form Beyotime; Incubation Time and Temperature: Overnight at 4 ℃ ; Secondary Antibody: Goat anti-Rabbit IgG-HRP antibody (HA1001); Dilution: 1:300,000 Dilution Buffer: 5% NFDM/TBST; Incubation Time and Temperature: 30 minutes at room temperature; Exposure time: 60/120 seconds. |
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Fig2: Competitive ELISA analysis of m6A was performed by coating wells of a 96-well plate with 50 µl per well of m6A -BSA diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 1%BSA blocking buffer, and incubated with 100 µl per well of m6A monoclonal antibody at concentration of 1 µg/mL with serial diluted m6A and its analogs starting from a concentration of 10,000ng/ml to 78ng/ml for 1 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-Rabbit IgG secondary antibody at a dilution of 1:15,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig3: Competitive ELISA analysis of m6A was performed by coating wells of a 96-well plate with 50 µl per well of m6A -BSA diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 1%BSA blocking buffer, and incubated with 100 µl per well of m6A monoclonal antibody at concentration of 1 µg/mL with m6A and its analogs at a concentration of 10ug/ml for 1 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-Rabbit IgG secondary antibody at a dilution of 1:15,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.