DM4 Recombinant Rabbit Monoclonal Antibody [3H3]
cat.: HA721153
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | ELISA |
| Clonality: | Monoclonal |
| Clone number: | 3H3 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | DM4 coupled with OVA. |
| Recommended Dilutions:
ELISA |
1:10,000 |
Images
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Fig1:
Anti-DM4 Antibody (HA721153) in indirect ELISA. Indirect ELISA analysis of DM4 was performed by coating wells of a 96-well plate with 50 µl per well of DM4-BSA diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with StartingBlock blocking buffer, and incubated with 50 µl per well of a mouse IL-2 monoclonal antibody starting at a concentration of 10 µg/mL and serially diluting it to a concentration of 0.169 ng/mL for 2 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:15,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 30 minutes at 37℃. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.