DM4 Recombinant Rabbit Monoclonal Antibody [3H3]
cat.: HA721153
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | ELISA |
| Clonality: | Monoclonal |
| Clone number: | 3H3 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | DM4 coupled with OVA. |
| Recommended Dilutions:
ELISA |
1:10,000 |
Images
|
Fig1:
Anti-DM4 Antibody (HA721153) in indirect ELISA. Indirect ELISA analysis of DM4 was performed by coating wells of a 96-well plate with 50 µl per well of DM4-BSA diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with StartingBlock blocking buffer, and incubated with 50 µl per well of a DM4 recombinant rabbit monoclonal antibody starting at a concentration of 10 µg/mL and serially diluting it to a concentration of 0.169 ng/mL for 2 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:15,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 30 minutes at 37℃. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2: Competitive ELISA analysis of DM4 was performed by coating wells of a 96-well plate with 50 µl per well of DM4-OVA diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 1%BSA blocking buffer, Serially diluted DM4 monoclonal antibody (HA721153), ranging in concentration from 4,000 ng/mL to 3.9 ng/mL, was added to the wells along with DM4 or DM4-OVA at a final concentration of 10 µg/mL each. The plate was incubated at room temperature for 1 hour. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-Rabbit IgG secondary antibody at a dilution of 1/15,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.