CD22 Recombinant Rabbit Monoclonal Antibody [PO00-31]
cat.: HA721157
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | PO00-31 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 95 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human CD22 aa 1-100 (N terminal). |
| Positive control: | Daudi (Human Burkitt's lymphoma cell) cell lysate, Raji (Human Burkitt's lymphoma cell) cell lysate, K-562 (Human chronic myelogenous leukemia cell) cell lysate, Jurkat (Human T-lymphoblastic cells) cell lysate, human B-cell lymphoma tissue, human tonsil tissue, human spleen tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P IF-Tissue |
1:1,000 1:1,000 1:200 |
| Uniprot #: | SwissProt: P20273 Human |
| Alternative names: | B cell receptor CD22 precursor B lymphocyte cell adhesion molecule B-cell receptor CD22 B-lymphocyte cell adhesion molecule BL CAM BL-CAM BLCAM CD 22 CD22 CD22 antigen CD22 molecule CD22 protein CD22_HUMAN Lectin 2 Leu14 Lyb8 MGC130020 sialic acid binding Ig like lectin 2 Sialic acid binding immunoglobulin like lectin 2 Sialic acid-binding Ig-like lectin 2 SIGLEC 2 Siglec-2 SIGLEC2 T cell surface antigen Leu 14 T-cell surface antigen Leu-14 |
Images
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Fig1:
Western blot analysis of CD22 on different lysates with Rabbit anti-CD22 antibody (HA721157) at 1/5,000 dilution. Lane 1: Daudi (Human Burkitt's lymphoma cell) cell lysate Lane 2: Raji (Human Burkitt's lymphoma cell) cell lysate Lane 3: K-562 (Human chronic myelogenous leukemia cell) cell lysate Lane 4: Jurkat (Human T-lymphoblastic cells) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 20 seconds; ECL: K1801 Negative expression of CD22 protein in K-562 and Jurkat is consistent with the predicted expression pattern. Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA721157, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 95 kDa Observed band size: 130 kDa |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human B-cell lymphoma tissue with Rabbit anti-CD22 antibody (HA721157) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721157) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD22 antibody (HA721157) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721157) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD22 antibody (HA721157) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721157) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Application: IF-Tissue Species: Human Site: Spleen Sample: Paraffin-embedded section Antibody concentration: 1/200 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.