CD47 Recombinant Rabbit Monoclonal Antibody [PD00-33]
cat.: HA721158
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | PD00-33 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 35 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human CD47 aa 50-150 (internal sequence). |
| Positive control: | Human ovarian tumor tissue, human prostate tissue, Jurkat. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
IHC-P FC |
1:1,000 1:500-1:1,000 |
| Uniprot #: | SwissProt: Q08722 Human |
| Alternative names: | Antigen identified by monoclonal 1D8 Antigenic surface determinant protein OA3 CD 47 CD47 CD47 antigen (Rh-related antigen, integrin-associated signal transducer) CD47 antigen CD47 glycoprotein CD47 molecule CD47_HUMAN IAP Integrin Associated Protein Integrin associated signal transducer Integrin-associated protein Leukocyte surface antigen CD47 MER 6 MER6 OA 3 OA3 OTTHUMP00000041152 OTTHUMP00000041153 Protein MER6 Rh related antigen Surface antigen identified by monoclonal 1D8 |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded human ovarian tumor tissue with Rabbit anti-CD47 antibody (HA721158) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721158) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human prostate tissue with Rabbit anti-CD47 antibody (HA721158) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721158) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Flow cytometric analysis of Jurkat cells labeling CD47. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721158, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.