Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human |
Applications: | IHC-P |
Clonality: | Monoclonal |
Clone number: | PD00-37 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 11 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within Human Gastrin (internal sequence). |
Positive control: | Human stomach tissue. |
Subcellular location: | Secreted. |
Recommended Dilutions:
IHC-P |
1:1,000 |
Uniprot #: | SwissProt: P01350 Human |
Alternative names: | Component II G14 G17 G34 G52 G6 GAS Gast GAST_HUMAN Gastrin Gastrin component I Gastrin component II Gastrin component III Gastrin precursor Gastrin-14 Gastrin-17 Gastrin-34 Gastrin-52 Gastrin-6 Gastrin-71 |
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Fig1:
Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-Gastrin antibody (HA721159) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721159) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human kidney tissue (negative) with Rabbit anti-Gastrin antibody (HA721159) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721159) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |