Gastrin Recombinant Rabbit Monoclonal Antibody [PD00-37]
cat.: HA721159
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | PD00-37 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 11 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Gastrin (internal sequence). |
| Positive control: | Human stomach tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
IHC-P IF-Tissue |
1:1,000 1:2,000 |
| Uniprot #: | SwissProt: P01350 Human |
| Alternative names: | Component II G14 G17 G34 G52 G6 GAS Gast GAST_HUMAN Gastrin Gastrin component I Gastrin component II Gastrin component III Gastrin precursor Gastrin-14 Gastrin-17 Gastrin-34 Gastrin-52 Gastrin-6 Gastrin-71 |
Images
|
Fig1:
Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-Gastrin antibody (HA721159) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721159) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human kidney tissue (negative) with Rabbit anti-Gastrin antibody (HA721159) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721159) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Application: IF-Tissue Species: Human Site: stomach Sample: Paraffin-embedded section Antibody concentration: 1/2,000 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.