CD13 Recombinant Rabbit Monoclonal Antibody [PD00-39]
cat.: HA721160
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | IHC-P, WB |
| Clonality: | Monoclonal |
| Clone number: | PD00-39 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 110 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within N Terminal human CD13. |
| Positive control: | Human tonsil tissue, human spleen tissue, human kidney tissue, mouse kidney tissue, PANC-1 cell lysate, THP-1 cell lysate, U-937 cell lysate, HL-60 cell lysate, Mouse kidney tissue lysate. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
IHC-P WB |
1:1,000-1:20,000 1:2,000 |
| Uniprot #: | SwissProt: P15144 Human | P97449 Mouse |
| Alternative names: | Alanyl (membrane) aminopeptidase Alanyl aminopeptidase Aminopeptidase M Aminopeptidase N AMPN_HUMAN ANPEP AP M AP N AP-M AP-N APN CD 13 CD13 CD13 antigen gp150 hAPN LAP 1 LAP1 Microsomal aminopeptidase Myeloid plasma membrane glycoprotein CD13 p150 PEPN |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD13 antibody (HA721160) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721160) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD13 antibody (HA721160) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721160) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-CD13 antibody (HA721160) at 1/20,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721160) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-CD13 antibody (HA721160) at 1/20,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721160) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Western blot analysis of CD13 on different lysates with Rabbit anti-CD13 antibody (HA721160) at 1/2,000 dilution. Lane 1: PANC-1 cell lysate (5 µg/Lane) Lane 2: THP-1 cell lysate (30 µg/Lane) Lane 3: U-937 cell lysate (20 µg/Lane) Lane 4: 293T cell lysate (negative) (20 µg/Lane) Lane 5: HL-60 cell lysate (20 µg/Lane) Lane 6: Mouse kidney tissue lysate (20 µg/Lane) Predicted band size: 110 kDa Observed band size: 150 kDa Exposure time: Lane 1: 9 seconds; Lane 2-5: 24 seconds; Lane 6: 1 minute 45 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721160) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig6:
Western blot analysis of CD13 on different lysates with Rabbit anti-CD13 antibody (HA721160) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-CD13 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 110 kDa Observed band size: 150 kDa Exposure time: 1 minute 2 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721160) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.