Uroplakin III Recombinant Rabbit Monoclonal Antibody [PD00-09]
cat.: HA721161
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: IHC-P, FC
Clonality: Monoclonal
Clone number: PD00-09
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 31 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Uroplakin III aa 250 to the C-terminus.
Positive control: Human bladder carcinoma tissue, Hela, 293.
Subcellular location: Endoplasmic reticulum membrane.
Recommended Dilutions:
  IHC-P
  FC

1:5,000
1:500-1:1,000
Uniprot #: SwissProt: O75631 Human
Alternative names: MGC119178 OTTHUMP00000028951 UP III UP3a UPIII UPIIIA UPK 3 UPK 3A UPK3 UPK3A UPK3A_HUMAN Uroplakin 3A Uroplakin III Uroplakin-3a Uroplakin3A UroplakinIII
Images
HA721161_1.jpg Fig1: Immunohistochemical analysis of paraffin-embedded human bladder carcinoma tissue with Rabbit anti-Uroplakin III antibody (HA721161) at 1/5,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721161) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721161_2.jpg Fig2: Flow cytometric analysis of Hela cells labeling Uroplakin III.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA721161, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
HA721161_3.jpg Fig3: Flow cytometric analysis of 293 cells labeling Uroplakin III.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA721161, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.